Time (h)

1
1
Absorbance (600 nm)
0.1
0.01
25
30
0
5
10
15
20
Time (h)
Figure S1. Effects of metD (abc-yaeE-yaeC)
operon expression in trans on growth of the
wild type, the ?abc-yaeE mutant and the ?yaeC
mutant in M9 minimal medium as described in
Figure 1. Ampicillin and arabinose were
added to the medium at 100 ?g/ml and 2
mM, respectively. ?, BW25113 (pBAD24) ?,
BW25113 (pBAD24-metD) ?, ?abc-yaeE (pBAD24) ?,
?abc -yaeE (pBAD24-metD) ?, yaeC (pBAD24) and
?, ?yaeC (pBAD24-metD).