Nanoparticle-Based Bio-Bar Codes

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Nanoparticle-Based Bio-Bar Codes

• Angela Wu
• October 12th, 2008

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Background

• Target Amplification for DNA (PCR)
• Interest for research, forensic and clinical
  applications
• Protein Targets?
• Improve medical diagnostics and proteomics

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Immuno-PCR

• Tag protein targets with oligonucleotide markers
• Amplify with PCR
• Use DNA detection to identify target

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Downfalls of Current Technology

• Low ratio of DNA sequence to Ab
• Slow target-binding kinetics
• Complex conjugation chemistries involved in
  linking DNA to Ab
• PCR requirements

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Bio-Bar Code Assay

• Magnetic microparticle probes with Ab
• Nanoparticle probes that are encoded with DNA and
  Ab that can sandwich the target
• Magnetic separation
• Dehybridization of the oligonucleotides
• Determination of presence of target protein by
  identifying sequence

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Bio-Bar Code Assay
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Advantages

• Target does not need to be immobilized
• Faster binding kinetics
• High ratio of PCR-amplifiable DNA to labeling Ab
• Increased sensitivity
• No complicated conjugation chemistry
• No additional Ab or DNA-protein conjugates

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Significance

• Ultrasensitive
• Proteins can be detected at 30 attomolar
  concentrations
• With PCR, at 3 attomolar
• Prostate-specific antigen (PSA) detection for
  prostate cancer and breast cancer

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Questions