My Favorite Pathway: Glycolysis/Gluconeogenesis

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My Favorite PathwayGlycolysis/Gluconeogenesis

• Pallavi Penumetcha
• 11/25/08

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Glycolysis (RAST)
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Glycolysis Pathway Condensed
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Example of How to Investigate One Enzyme
Enzyme 3.1.3.11 was not highlighted in the SEED
pathway
CDD Results
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Pfam Results
NCBI Results
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Enzymes not called by RAST annotation
EC Number and Name Other Databases? Notes
3.1.3.11 (fructose bisphosphatase) Yes SEED Confirmed as fructose bisphosphotase in CDD, Pfam and NCBI
2.7.1.11 (6-phosphofructokinase) Yes SEED and Manatee 5 calls between 2 databases (2 in SEED and 3 in Manatee) 2 sequences in SEED match with two in Manatee?call different EC numbers -SEED 2.7.1.11 -Manatee 2.7.1.56 (1-phosphofructokinase) 1 sequence different in Manatee 2 sequence match same results in CDD Sequence in Manatee has the superfamily related to phosphofructokinases
1.2.1.12 (glyceraldehyde-3-phosphate dehydrogenase, phosphorylating) Yes All three All three have same protein sequence SEED calls the EC number 1.2.1.59 (glyceraldehyde-3-phosphate dehydrogenase NADP, phosphorylating) Shows that the different databases have different criteria for calling a protein
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Fructose and Mannose Metabolism
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Pentose Phosphate Pathway