Light Microscopy

1
Light Microscopy

Bright Field
Phase-Contrast
Differential Interference Contrast (DIC)

Fluorescence
2
Electron Microscopy (EM)
Scanning EM
Transmission EM
3
Chemical Composition of Cells
Pg. 58
4
Pg. 79
5
Chemical Bond Energies
6
Gene Expression

7
Monomer/Polymer
8
Nitrogenous Bases of DNA and RNA
Pg. 76
9
Pg. 175
10
(No Transcript)
11
5
3
5
3
12
Major Groove
Minor Groove
Pg. 171
13
Supercoiled DNA
Non-supercoiled DNA (relaxed)
14
Werner Syndrome
15 yrs. 48
yrs.
Mutation in gene encoding RecQ helicase
15
Pg. 175
16
5
3
5
3
17
Human Karyotype
Pg. 179
18
RNA Molecules
5
5
3
3
19
(No Transcript)
20
Pg. 74
21
Amino Acid Side Chains
(Pgs. 74-75)
22
(No Transcript)
23
(No Transcript)
24
Extra carbon atom!
25
alpha-helix
ß-Sheet
26
Hydrophobic Interactions in Tertiary Structure
27
Representations of Protein Tertiary Structure
Wire Frame
Backbone
Space Filling
Ribbon
Pg. 132
28
Quaternary Structure
Pg. 141
29
Chloride Ion Channel Protein
30
Comparison of Protein Primary Structure
Cyclin-dependent Kinase Proteins
31
(No Transcript)
32
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33
(No Transcript)
34
Gene Expression

Pg. 230
35
RNA Synthesis
OH
OH
OH
Pg. 202
36
Pg. 233
37
1 µm
Pg. 233
38
Fig. 7-20
39
Exon/Intron Organization of Eukaryotic Genes
Pg. 238
40

41
Sequence Structure of Introns
42
Pg. 239
43
Alternative Splicing
Pg. 241
44
5 Guanine Cap Structure

Pg. 237
45
Cytosol Nucleus Mitochondria
Secreted ER Golgi Endosome Lysosome Transmembrane
46
(No Transcript)
47
(No Transcript)
48
Reading Frames in RNA
49
(No Transcript)
50
(No Transcript)
51
tRNA Synthetase
Editing Domain
Amino Acid
tRNA
Synthetase (blue)
52
Editing by tRNA Synthetase
53
(No Transcript)
54
Large Subunit RNA Secondary Structure
55
Small Ribosomal Subunit
Pg. 253
56
mRNA Structure
3
57
Initiation of Translation
Pg. 253
58
Pg. 252
59
Release Factor (RF)
tRNA
60
(No Transcript)
61
Co-translational Protein Folding
62
Protein Folding by Chaperones
63
(No Transcript)
64
(No Transcript)
65
Transcriptional Repressor
Transcriptional Activator
66
Ubiquitin-dependent Protein Degradation
Pg. 257
67
(No Transcript)
68
Coding and catalysis
Coding
Coding intermediate
69
Fungal Disease
Systemic Infections
Superficial Infections
70
Onochomycosis
71
AN2690 Structure
72
Figure 1A
73
Figure 1B
74
Figure 3
AN2690