Nanoparticle-Based Bio

1
Nanoparticle-Based BioBarCodes for the
UltrasensitiveDetection of Proteins
SCIENCE VOL 301 26 SEPTEMBER 2003
Jwa-Min Nam, C. Shad Thaxton, Chad A. Mirkin

• Qing Han
• 20.309 Presentation
• 10.14.08

2
Background
PCR
Immuno-PCR
ELISA
SensitivitypM
3
Drawbacks of current immuno-PCR

• A low ratio of DNA to detection Ab
• Slow target-binding kinetics
• Link the Ab and DNA markers
• PCR requirements

4
The bio-bar-code assay method
-----Probe designand preparation.
Detection Ab
Capture Ab
1 um
5
PSA detection and bar-code DNA amplification and
identification
6
Scanometric detection of PSA-specific bar-code DNA
7
Biobar-code method offers several advantages

• Homogeneous target-binding portion
• High ratio of PCR-amplifiable DNA to Ab
• No need for complicated conjugation chemistry
• Low background signal
• potential for the simultaneous detection of many
  analytes in one solution

8
Questions?