flow analysis

1
Oxidative Burst
DCFH-DA
DCFH-DA
DCFH
H O
2 2
2
Fluorescent Dyes
3
Fluorescent Dyes
Wavelength
400 nm
600 nm
700 nm
500 nm
Excitation
Emission
Phycoerythrin (PE)
4
Immunophenotyping
CD2
CD4
5
Immunophenotyping
1000
100
10
1
.1
Log FITC
6
CD4/CD8 Quadstats
1
2
45
2
3
4
27
26
.1
1
10
100
1000
Log FITC Fluorescence (CD8)
7
Normal Cell Cycle
M
G0
G2
DNA Analysis
G1
s
Count
s
0
200
400
600
800
1000
DNA content
8
DNA Analysis
0
200
400
600
800
1000
4N
2N
PI Fluorescence
9
DNA Analysis
Aneuploid peak
0
200
400
600
800
1000
PI Fluorescence
10
Chromosome Analysis
(Bivariate Flow Karyotyping)
chromosome 1
chromosome 2
11
Reticulocyte Analysis
RMI 34
RMI 0
R1
R3
R2
R4
R3
R1
R2
R4
1000
1000
.1
100
1
10
1
10
100
.1
log Thiazole Orange
log Thiazole Orange
12
Calcium Flux - Indo-1
13
Light Scatter Gating
Side Scatter Projection
Neutrophils
Scale
1000
200
100
50
40
Monocytes
30
20
15
Lymphocytes
8
200
400
600
800
1000
0
90 Degree Scatter
14
Phagocytic Vacuole