In silico feedback for in vivo regulation of a gene expression circuit

1
In silico feedback for in vivo regulation of a
gene expression circuit

• Shared by lxshen

2
Biological feedback

• Signals
• Biological control circuits
• Implement of control

3
Comparison of control circuits

• Synthetic biological circuits

• Electronic circuits

• Prosperous
• Easy to control
• More functions
• Fast

• Nature circuits robust
• Difficulty in artificial circuits
• Not easy to control
• slow

4
Interface
5
model

• Fourth-order linear ordinary differential
  equation
• Identification experiment to determine five
  parameters

fluorescence delay (fluorescence ? gene
expression)
6
Applying model ?

• Task increasing fluorescence 4 or 7 times over a
  7-h period
• Open-loop(OL)control
• Fail to regulate fluorescence to desired levels
• due to inaccuracies of the model and something
• unpredictable

7
Applying model ?

• Closed-loop(CL) feedback control
• Kalman filter

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Significance

• More predictable and accurate
• Avoid the disadvantage of bio-circuits
• Facilitate synthetic systems

9

• Q?A