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Supplemental material Figure 1_ 2D-resolution
of water-soluble proteins from the leaf apoplast
of the two cowpea cultivars TVu 91(A1/A2) and TVu
1987 (B1/B2) differing in Mn tolerance by
IEF/SDS-PAGE. Plants precultured for 14 days were
treated with 50 µM Mn for 5 days (A2, B2), while
control plants received 0.2 µM Mn continuously
(A1, B1). Numbers on the top of the gels indicate
the pH gradient, and the numbers on the left
indicate molecular masses of standard proteins.
Arrows indicate identified proteins (the numbers
correspond to proteins identified in
Fecht-Christoffers et al., 2003b, Fig. 7, Table
II), e.g. pathogenesis related like proteins
(spot 2, 8 and 10), chitinase (spot 13, 14 and
16), peroxidase (spot 16 and 18), thaumatin-like
protein (spot 18) and glucanase (spot 18 and 20).