BASICS OF LABORATORY SAFETY

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BASICS OF LABORATORY SAFETY

• Introduction to Microbiology Laboratory
• Donna C. Sullivan, PhD

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Before Beginning Any Laboratory Exercise

• Know the dangers associated with all materials
  you will use in this laboratory exercise
• Know the proper way of using all the materials
  provided for the exercise
• Know all the protective measures you must use
  during the exercise

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Personal Protection

• Wear an apron or lab coat in the proper manner
• Wear gloves in the proper manner
• Wear a face shield in the proper manner

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Personal Protection

• Tie long hair neatly at the back of the neck
• Wear shoes that enclose your entire feet

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Emergency Procedures

• Know where all exit doors are located
• If a reagent splashes onto your face or eyes,
  wash your face at the eyewash station for several
  minutes

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Emergency Procedures

• If an irritating or harmful reagent splashes onto
  your body, use the emergency shower to wash your
  body for several minutes
• Know where the fire extinguisher and fire blanket
  are located

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Emergency Procedures

• If a reagent spills onto the workbench or floor,
  notify the instructor immediately for cleanup
  instructions.
• If a glass container gets broken, notify the
  instructor immediately for cleanup instructions.
• Report all accidents, injuries and close calls
  immediately to the instructor.

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Good Laboratory Conduct

• Follow all instructions
• Do not play in the laboratory
• Do not use any laboratory equipment until you
  are told to do so
• Do not perform any laboratory procedure until you
  are told to do so

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Good Laboratory Conduct

• Do not bring food, beverages or tobacco products
  into the laboratory
• Do not apply cosmetics in the laboratory
• Do not eat or drink in the laboratory
• Restrain loose clothing and jewelry

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Before You Leave The Laboratory

• Clean your workbench with disinfectant at the
  beginning and at the end of the laboratory
  exercise
• Leave all equipment, samples and reagents in the
  lab
• Leave your lab coat (apron) in the lab
• Wash your hands with soap and water

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COMPOUND MICROSCOPE
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COMPOUND MICROSCOPE
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COMPOUND MICROSCOPE
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COMPOUND MICROSCOPE
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Compound Light Microscope
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Using the Microscope

• To carry the microscope grasp the microscopes arm
  with one hand. Place your other hand under the
  base.
• Place the microscope on a table with the arm
  toward you.
• Turn the coarse adjustment knob to raise the body
  tube.
• Revolve the nosepiece until the low-power
  objective lens clicks into place.

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Total Magnification
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PREPARING WET MOUNTS
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PREPARING WET MOUNT
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ADJUSTING MICROSCOPE