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?????????,????CC?????-????-?????????2.5?????
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(No Transcript)
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(No Transcript)
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• ???
• (Restriction enzymes)
• - ????????, ????????
• ??? (DNA ligase)
• ??DNA (recombinant DNA)

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Genetic Engineering (????)
???? ???
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???????????(vector)?
(Vector ??? Plasmid ??????)
???? ???
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• ???????????????????
• ???????????,????????(probe) ,??? DNA
  ????(hybridization)??,???????????,??????????????.

colonies containing gene of interest
???? ???
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DNA???(DNA Libraries) ??????(genomic library)
DNA fragments from source DNA
???? ???
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????????(Restriction Fragment Length
Polymorphisms ,RFLP) ???DNA??
???????
??????????
?????
Wiki Mnolf
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• ?????????,????,????, ??????

???? ???
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• ???????
• ??????????DNA ??E coli???????????
• ?????????? E coli

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??????? ? Ti Plasmid ?DNA??
???? ???
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????????DNA??,???????????,????????????????,???????
????????????
???????
???? ???
????????????
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???????????

• ????????????
• insert genes encoding proteins harmful to insects
• ??????????
• ??????????????????

Wiki Skryabin BV et al.
Flickr Dimitry B
Wiki Skryabin BV et al.
Wiki Urville Djasim
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• ????????????? ( transgenic rice) ????????70
  ??????Vit. A ???

???????
GMO (???????)
Wiki Peter Beyer
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?????????
(Wilmuts Animal Cloning Experiment)
????? (?????)
??????? (??????)
???????? (???2N???)
????????
??????????????? ?????????
????????(2N)????????
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??????????,????????????????????? Q
????????????? A ???, B ???, C????
????
????
Wiki Toni Barros
????????
??????
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???(blastula) - ????????????
???? (ICM)
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????
???
???????? (stem cells)
???????????????? (??????)
???????? (???, ???)
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????? (ESC) ????????
Wiki Mike Jones
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???????????????????,???????,??????????????????????
?,?????????
Wiki Mike Jones
??????,?????
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????

• ??????????????????
• GMO????????? ???
• ??????????????????
• ???????????????? ????
• ????????????????
• ??????????,?????????????????????
• ??????????
• ????? ?????

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???????(cloned)??

• ??????????
• ?????(ESC)
• ????????????
• ???

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????

• ???????(ESC)??????,??????????ESC,??,?????,????,???
  ????
• ???????????,???????

?????
???????
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????

• ???? (Genetic solution) (Discovery)
• ???????????
• ?????????????????? ????????????

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????
?? ???? ??/??
Wiki Cnickelfr http//commons.wikimedia.org/wiki/FileChromosome.gif 2010/05/08 visited
Flickr kat m research http//www.flickr.com/photos/36128932_at_N03/3339221708/ 2010/05/08 visited
Wiki Boumphreyfr http//commons.wikimedia.org/wiki/FileDna_strand.png 2010/05/09 visited
Wiki user AutisticPsycho2 http//en.wikipedia.org/wiki/FileDNA-structure-and-bases.png 2010/05/09 visited
Wiki Adenosine http//en.wikipedia.org/wiki/FileConjugation.svg 2010/06/11 visited
Wiki SeanMack http//en.wikipedia.org/wiki/FileDNA_Under_electron_microscope_Image_3576B-PH.jpg 2010/06/11 visited
Wiki Tinastella http//commons.wikimedia.org/wiki/FileRestriction_enzyme_Eco_RI.JPG 2010/06/19 visited
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????
?? ???? ??/??
Wiki Mnolf http//commons.wikimedia.org/wiki/FileGel_electrophoresis_2.jpg 2010/06/19 visited
Wiki Retama http//commons.wikimedia.org/wiki/FileRFLP_mapping.svg 2010/06/19 visited
Wiki Karel Schmiedberger ml http//commons.wikimedia.org/wiki/FileAnimal_cells_cultivation_in_fermenter.JPG 2010/06/20 visited
Wiki Skryabin BV, Gubar LV, Seeger B, Pfeiffer J, Handel S, et al. http//commons.wikimedia.org/wiki/FilePWS_mice.jpg 2010/06/20 visited
Wiki Urville Djasim http//commons.wikimedia.org/wiki/FileWLANL_-_Urville_Djasim_-_Stier_Herman_-_Herman_the_Bull_(2).jpg/2010/06/20 visited
Flickr Dimitry B http//www.flickr.com/photos/ru_boff/3403148157/ 2010/06/20 visited
Wiki Squidonius http//en.wikipedia.org/wiki/FileDolly_clone.svg 2010/06/20 visited
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????
?? ???? ??/??
Wiki Toni Barros http//commons.wikimedia.org/wiki/FileDolly_face_closeup.jpg 2010/06/20 visited
Wiki user GBuilder http//en.wikipedia.org/wiki/FileCell_differentiation.gif 2010/06/20 visited
Wiki Mike Jones http//commons.wikimedia.org/wiki/FileStem_cells_diagram.png 2010/06/20 visited
Wiki Peter Beyer http//commons.wikimedia.org/wiki/FileProf_Peter_Beyer.jpg 2010/06/20 visited
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