Master

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Master the Microscope
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What type of microscope are we using?
Compound
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Compound Microscope

• A compound microscope consists of an objective
  lens and an eyepiece at opposite ends of an
  adjustable tube.

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nosepiece
eyepiece
high-power objective lens
arm
stage
course adjustment knob
scanning objective lens
fine adjustment knob
low-power objective lens
diaphragm
base
stage clips
light source
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Arm and Base

• These two pieces are used to tilt, lift, support
  and carry the microscope carefully.

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Light Source

• This part provides light to view the specimen.

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Course Adjustment Knob

• This knob moves the body tube or stage
  (depending on your microscope) up and down.

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Stage

• This is the area to place your specimen for
  viewing.

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Diaphragm

• This part of the microscope regulates the amount
  of light that enters through the stage opening.

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Stage Clips

• These hold the slide in position.

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Nosepiece

• This piece contains all of the objective lenses.

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Fine Adjustment Knob

• This is a knob that helps to focus on fine
  details of the specimen.

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Eyepiece

• This is the piece that you look through to see
  the specimen.

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Objective Lenses

• These pieces enlarge the specimen to scanning,
  low, and high level of magnification.

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Now we know all of the parts, but how do we use
the microscope?
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Step 1

• To carry the microscope grasp the microscopes arm
  with one hand.
• Place your other hand under the base. Place the
  microscope on a table with the arm toward you.
• Plug the microscope in and turn on the light
  source or adjust the mirror. Make sure that the
  cords do not obstruct walking paths.

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Step 2

• Turn the coarse adjustment knob to lower the
  stage.

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Step 3

• Revolve the nosepiece until the scanning
  objective lens clicks into place.

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Step 4

• Adjust the diaphragm so that it lets in the most
  light possible.
• Look through the eyepiece to assure that all
  parts are correctly aligned.

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Step 5

• Place a slide on the stage. Center the specimen
  over the opening on the stage. Use the stage
  clips to hold the slide in place.

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Step 6

• Look at the stage from the side. Carefully turn
  the coarse adjustment knob to raise the stage
  until the scanning power objective almost touches
  the slide.

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Step 7

• Looking through the eyepiece, VERY SLOWLY turn
  the coarse adjustment knob to lower the specimen
  until it comes into focus.

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Step 8

• To switch to the high power objective lens, look
  at the microscope from the side. CAREFULLY rotate
  the nosepiece until the high-power objective lens
  clicks into place. Make sure the lens does not
  hit the slide.

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Step 9

• Now switch to the low power objective lens, look
  at the microscope from the side. CAREFULLY rotate
  the nosepiece until the low-power objective lens
  clicks into place. Make sure the lens does not
  hit the slide.

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Step 10

• Looking through the eyepiece, turn the fine
  adjustment knob until the specimen comes into
  focus.

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Step 11

• Diagram your observation through each of the
  objective lenses.

Wowthis is huge!
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You are now a
Master of the Microscope