Pr

1
A
B
90
2C
80
4C
70
8C
60
16C
DNA content
50
40
30
20
10
0
8D
17D
Supplemental Figure S1 (A) H4 gene expression in
8- and 17-d-old Arabidopsis plantlets. RT-PCR was
performed on RNR samples extracted from 8-d-old
(8D) and 17-d-old seedlings (17D) using H4
specific primers and 18S ribosomal RNA gene
primers as a standard (Supplemental, Table 1).
Thirty cycles of PCR reaction were performed. PCR
products were analyzed on agarose gel. (B)
Evaluation of pourcentage of 2C, 4C, 8C and 16C
DNA content by flow cytometry in the 8 and
17d-old plantlets. SDs are indicated.