?????(Flow Cytometer) ???? PowerPoint PPT Presentation

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Title: ?????(Flow Cytometer) ????


1
?????(Flow Cytometer)????
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  • ??????????????
  • 2005?3? ???

2
????
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3
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BD FACSVantage
BD-Calibur
4
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5
?FCM???
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6
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7
(No Transcript)
8
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(3)???(????). ??150,000?/ ?
??100,000?/?
9
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70????????????? ?? Becton-Dickson Coulter-Beck
man DOCytomation ?????????????
10
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11
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12
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?????,??,?????PMT??????????
13
????
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FSC SSC FL1 FL2 FL3
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????,????
(??,??,??)
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14
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15
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16
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???????????????
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?????????? ???????????,???????
17
PIAnnexin-V??????
DNA??-PI
Annexin-V
18
PEFITC???????????????
?????(linear)
????? (linear)
19
  • ????

20
??????????
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  • ??????????????
  • ????0.2uM-300uM?
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21
  • ???????????????????
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  • ?????H(???PH?),Na,K,Ca2(????
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  • ??DNA??,DNA??,DNA??,RNA,??????
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22
???? ??? ???? ???? ???? ??? ??????? ?????
..
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23
??????
???
DNA ??
G1
S phase
M
G0/1
G2
G1
G2/M
S
1
2 1
DNA??
??????
24
???????
2
1
4
3
?????
5
1 / 3
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Hoechst 33258 (AT)
X 8
7 recip 9-22
13
Y 18
9-12
14 15 16 17
20
21
19
22
Ph
Chromomycin A3 (GC)
25
????????????
  • ??BD FACSVantage SE
  • ????10,000?/?,??7,000?/???488nm,UV???????
  • ??1406
  • ??????64839306 ? 64862503
  • ????lcc_at_moon.ibp.ac.cn
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