bone

1
Osteoblasts
bone formation assay
t.arnett_at_ucl.ac.uk
2
Trabecular bone nodules formed by rat primary
osteoblasts cultured 14 days
5 mm
Unstained preparations
3
Dystrophic mineralisation of osteoblast cell
layers
in presence of 10 mM ?-glycerophosphate ? cell
death inhibition of normal bone matrix
formation
4
Discrete mineralisation of trabecular bone
matrix formed by rat osteoblasts cultured with 2
mM ?-glycerophosphate
Alizarin red stain for calcium
5
Alkaline phosphatase staining and matrix
mineralisation in primary rat osteoblast cultures
6
Hypoxia inhibits osteoblast proliferation and
bone formation
7
Acidosis blocks mineralisation of bone matrix
nodules formed by rat osteoblasts
pH 7.4
Alizarin red stain for calcium
pH 6.9