HIV-1/HIV-2 COMBINATION SCREENING

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HIV-1/HIV-2 COMBINATION SCREENING AND OUTCOMES
OF FOLLOW UP William R. Oleszko,
Ph.D. Associate Director, Public Health
Laboratory Department of Health and Mental
Hygiene New York City
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HIV ANTIBODY TESTING 2004 April 2004
Initiated use of new HIV antibody screening
assay BioRads HIV-1/HIV-2 Plus O
EIA April December 2004 73,955 specimen
submissions 96.5 NEGATIVE for antibody to
HIV-1/HIV-2 3.3 POSITIVE for antibody to
HIV-1 0.2 INCONCLUSIVE for antibody to
HIV-1
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HIV-2 ANTIBODY
YEARS STUDY SUBJECT AVERAGE HIV-2 CASES/YEAR AVERAGE SUBMISSIONS/YEAR
1988 1993 Prospective HIV-1 Inconclusive 5 158,959
1994 1997 Retrospective HIV-1 Inconclusive AOB Africa 12 156,020
1998 2004 Prospective All Submissions 38 120,200
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CATEGORIES FOR SPECIAL STUDY Atypical
but reactive HIV-1 Western blot banding pattern
Inconclusive pregnant or two HIV-1
inconclusives within one month Area of
birth Africa/Asia HIV-2 reactivity
AIDS symptoms and HIV antibody negative
Child of HIV positive mother HIV
Positive, no ART, undetectable viral load,
declining CD4 HIV positive, on ART,
undetectable viral load, declining CD4
OraQuick preliminary positive confirmatory result
inconclusive
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ASSAYS PERFORMED ON SPECIAL STUDY
SPECIMENS Current HIV-1/HIV-2 Multispot
Rapid EIA HIV V3 serotype CD4/CD8 T-cell
Enumeration HIV-1 DNA PCR HIV-2 DNA
PCR Viral load - HIV-1 RNA PCR HIV-1
bDNA Future HIV Genotyping/Resistance
Testing
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SEEK AND YE SHALL FIND!
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Public Health Laboratory PHL Sara T. Beatrice,
Ph.D., Assistant Commissioner Retrovirology
Laboratory Amado Punsalang, Jr., Ph.D. Robert
Pirillo Community Coordinator Mona El-Fishawy
- HIV Screening Lab Mohammad Younis HIV
Supplemental Testing Lab Ed Lee. Ph.D. HIV PCR
Lab Orlin Trochev, MD, Ph.D. HIV
Genotyping/Resistance Lab