Supplementary Figure 1

1
Supplementary Figure 1
y 13.018 - 0.0482x R2 0.99152
Supplementary Fig.1 Standard curve by survival
days and contents of inoculated mBSE-BH in ddY
mice. This figure shows the relationship
between survival days and contents of inoculated
mBSE-infected brain homogenate in ddY mice. We
estimated the infectivity titers of samples by
the incubation interval between 154 days and 235
days according to this standard curve. The
average incubation interval of mono-culture group
2 (256.4 days) is out of the detection range.
That of mono-culture group 3 (234.8 days) is also
very close to the detection limit. Only the titer
of mono-culture group 1 (227.4 days) is estimated
at 101.8 LD50 / ml somehow. In contrast, the
averages of incubation interval of all co-culture
groups are on the calculating range, and the
minimum titer of co-culture groups is estimated
at 103.3 LD50 / ml. These results indicate that
the infectious titer of basal medium of
co-cultures is at least gt30-fold higher than that
of mono-cultures.