Mendel

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• Mendels Contribution(s)?

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This Week

• Finish C. elegans experiment,
• Tinker with wasps,
• Exam Review (April 20th)
• Exam I (April 23rd),
• Basic Mendel!
• Basic Mendel!
• Basic Mendel!
• Basic Mendel!
• Basic Mendel!
• C. elegans!
• Bioinfomatics!
• Nasonia!

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Mendels First Postulate

• Unit Factors in Pairs
• Genetic characteristics are controlled by unit
  factors that exist in pairs in individual
  organisms,
• each individual receives one unit factor from
  each parent,
• in a monohybrid cross, three combinations of unit
  factors are possible,

not in Nasonia males!
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Mendels Second Postulate

• Dominant/Recessive
• When two unlike unit factors are present in a
  single individual, one unit factor is dominant to
  the other, which is said to be recessive.

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Mendels Third Postulate

• Segregation
• During the processes of heredity, the paired unit
  factors separate so that the offspring receives
  one unit factor from each parent,
• The unit factors segregate to offspring randomly.

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Mendels Forth Postulate

• Independent Assortment
• How do two traits segregate in the offspring of
  an individual that is heterozygous for both
  traits?

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Independent Assortmentchecking complementation
in the F2

• P
• F1
• F2

• AAdd x aaDD
• AaDd
• 9 A_D_
• 3 aaD_
• 3 A_dd
• 1 aadd

P F1 F2
aadd x AADD AaDd 9 A_D_
3 aaD_ 3 A_dd 1 aadd
Both Recessive? Both Dominant? One of each?
97 wt mut
115 wt mut
313 wt mut
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P to F1Haplo/Diplo Thinking about
complementation groups

• Both recessive aaDD x Ad ? female AaDd,
  male aD
• Both Dominant aaDD x Ad ? female AaDd, male
  aD
• One dominant aadd x AD ? female AaDd, male
  ad
• or AADD x ad ? female AaDd, male
  AD
• need to define
  which is dominant!

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P to F1 to F2Haplo/Diplo Thinking about
complementation groups

• Both recessive aaDD x Ad ? female AaDd ?
  ¼AD ¼Ad ¼aD ¼ad
• Both Dominant aaDD x Ad ? female AaDd ?
  ¼AD ¼Ad ¼aD ¼ad
• One dominant aadd x AD ? female AaDd ?
  ¼AD ¼Ad ¼aD ¼ad
• or AADD x ad ? female AaDd ? ¼AD ¼Ad
  ¼aD ¼ad
• need to define
  which is dominant!

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Forked-Line MethodHaplo/Diplo F2AaDd x AD
(male genotype could be ad, aD, Ad)
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Forked-Line MethodHaplo/Diplo F2AaDd x AD
(male genotype could be ad, aD, Ad)
x 1 AD ¼ AADD
x 1 ad ¼ AaDd
x 1 AD ¼ AADd
x 1 ad ¼ Aadd
x 1 AD ¼ AaDD
x 1 ad ¼ aaDd
x 1 AD ¼ AaDd
x 1 ad ¼ aadd
Have Fun!
x 1 aD ¼ AaDD
x 1 Ad ¼ AADd
x 1 Ad ¼ AAdd
x 1 aD ¼ AaDd
x 1 aD ¼ aaDD
x 1 Ad ¼ aaDd
x 1 Ad ¼ Aadd
x 1 aD ¼ aaDd
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Lab Life Cycles

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Wasp Development TimelinesLife Cycles, Mating,
etc.
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C. elegans Nomenclature
Tower of Babel
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This Week

• Wednesday,
• score F3s,
• discuss Mendel Review Formal Lab Write-Up,
• think about or tinker with wasps?
• Friday,
• midterm,
• think about or tinker with wasps?