Therapeutic Treatment of Resveratrol on Alzheimer

1
Therapeutic Treatment of Resveratrol on
Alzheimers Disease with CL2006

• Sohrab Vatsia

2
Need
Alzheimers Incidents in the U.S.
Figure 1
Figure 2
http//uscnews2.usc.edu/Publication_JPGS/HSCWeekly
/2003/HSCW2665p1.jpg
http//www.omh.state.ny.us/omhweb/Statewideplan/20
05/images/figure4.2.gif
3
Alzheimers Disease
Figure 1
Figure 2
Figure 1
http//www.nia.nih.gov/NR/rdonlyres/A294D332-71A2-
4866-BDD7-A0DF216DAAA4/0/Alzheimers_Disease_Unrave
ling_the_Mystery.pdf
http//www.nia.nih.gov/NR/rdonlyres/A294D332-71A2-
4866-BDD7-A0DF216DAAA4/0/Alzheimers_Disease_Unrave
ling_the_Mystery.pdf
Figure 3
http//www.nia.nih.gov/NR/rdonlyres/A294D332-71A2-
4866-BDD7-A0DF216DAAA4/0/Alzheimers_Disease_Unrave
ling_the_Mystery.pdf
4
Resveratrol
http//www.resveratrol.org.uk/resveratrol.gif

• Rogers, Kara, ed. "Alzheimer's Disease."
  Encyclopedia Britannica. 15th ed. 2002. 306-07.

5
Resveratrols Role
http//www.chiro.org/nutrition/ABSTRACTS/Resveratr
ol_in_Prevention_and_Treatment.pdf
6
CL2006
http//wormsrus.50webs.com/Nematodes/C20elegans.J
PG
7
Literature Review

• Marambaud, 2008

(Marambaud, 2008)
8
Literature Review

• Vingtdeux, 2005

(ONeill, 2002)
9
Literature Review

• Anekonda, 2006

(Rodgers, 2006)
10
Literature Review

• Duchen, 2004

(Marambaud, 2005)
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Hypothesis/ Purpose

• The purpose of the experiment is to analyze the
  therapeutic and preventative potential of
  Resveratrol on Alzhemiers disease.
• Alternate - with the administration of
  Resveratrol in the CL 2006 model, amyloid-beta
  production will slow, and the spread of the
  amyloid-beta molecules will reduce.
• Null - There will be no change in amyloid beta
  production nor paralysis with the addition of
  Resveratrol.

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Protocols

• Preparation of Bacterial Plates (Siernagle, 2009)

(Yun, 2007)
13
Protocols

• Resveratrol Administration (Halliwell, 2004)
• Concentration ltor to 100 micromolars

(Lenker, 2001)
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Protocols

• C. elegans synchronization
• Paralyzed versus Non-Paralyzed

(Cheng,2002)
15
Protocols

• Staining using Congo Red Dye-registers Amyloid
  Beta Plaques

(Zhao, 1999)
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Do-Ability

• This project is do-able because we have all the
  necessary materials in the lab
• C. elgegans (both CL 2006 and wild-type) are
  readily available as well as their environments
  ie plates, food, living conditions
• Resveratrol is also readily available, it can be
  purchased relatively easily
• Amyloid Stain is readily available
• Microscope/s are available along with the prism
  and a Motic camera are also readily available
  thus observation is do-able

17

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C. Elegans Control
CL 2006 Control
CL 2006 Resveratrol (Preventative)
CL 2006 Resveratrol Therapeutic
CL 2006 Gamma-Secretase
C. Elegans adminstered Resveratrol(negative
control)
Stain and monitor amyloid levels, as well as
monitor lifespan
Treat on the onset of paralysis. Measure
lifespan, stain, and measure amyloid-beta levels
Monitor Life Span, Stain, and obeserve
amyloid-beta levels
Tested in order to make sure that the
amyloid-beta is being targeted (2 or 3 trials)
Treat with Resveratrol prior to paralysis.
Measure Lifespan, stain,and measure amyloid-beta
levels.
Monitor Lifespan
Record Qualitative and Quantitative Data based
upon the observations, and following the
protocols, (Lifespan, Amyloid-Beta Levels)
Statistical Analysis by Two Way Anova followed by
Scheffe Post-Hoc (plt.05)
19
Bibliography

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