An overview of the activities of our team

1
An overview of the activities of our team

• Authors
• Rosa Graciela Cohen
• Alba Graciela Papeschi
• Ximena Inés Pastorino
• Francisco Amat

2
(No Transcript)
3
(No Transcript)
4
Lagoon in Comandancia Frías (Chaco Prov.)
24º 30S, 62º 15W Salinas Grandes (NO Córdoba
Prov.) 26º 30-31º 40S, 64º 15-66º
25W Lagoon 40km Northern Los Juríes (Santiago
del Estero Prov.) 28º 03S, 62º 10W La
Antigua Saline (La Rioja Prov.) 29º 54S,
66º 07W Mar Chiquita Lake (Córdoba Prov.)
30º 30- 31º S, 62º 10- 63º 10W
Pampa de las Salinas (San Luis-La Rioja Prov.)
32º S, 66º 41 W Las Tunas Lagoon
(Córdoba Prov.) 33º 44S, 62º 32W Lagoon
in Trenque Lauquen (Buenos Aires Prov.)
35 48S, 62
55W Lagoon in Área de Naicó (La Pampa Prov.)
approximately
36º52S, 64º 24W Salinas Grandes de Hidalgo
(La Pampa Prov.)
37º 13S, 63º 26W Utracán
Lagoon (La Pampa Prov.)
37º 1714S, 64º
3714W Guatraché Lagoon (La Pampa Prov.)

37º4425S, 63º 3315W El Carrizal Lagoon
(La Pampa Prov.)
37º 5048S, 63º 4654W
Colorada Grande Saline or Lagoon (La Pampa
Prov.)
38 18S, 63 42W Colorada Chica Saline or
Lagoon (La Pampa Prov.)
38º 23S, 63º 36W Callaqueo
Saline or Lagoon (La Pampa Prov.)
38º 34S,
63º 32W Salitral de la Vidriera ( de
Garnica) (Buenos Aires Prov.)
38 42S, 62 40W Salinas Chicas
(Buenos Aires Prov.)
38º 44S,
62º 57W Lagoon in San Miguel Field ( of
Winchel) (Buenos Aires Prov.)
approximately 39ºS, 62º 30W Palos Blancos
Saline or Lagoon (Buenos Aires Prov.)
approximately 39ºS, 62º 30W
Lagoon in Hotel Las Termas (Pedro Luro, Buenos
Aires Prov.) 39 30S,
6235W Villalonga Lagoon (de los Gauchos)
(Buenos Aires Prov.)
39 51S, 62 32W Salitral and Saline of
Bajo del Gualicho (Río Negro Prov.)
40º 24S, 65º 13W Salina de
Luzzetti (Buenos Aires Prov.)
approximately 40º 35S, 62º
40W Salina del Algarrobo (Buenos Aires Prov.)

40 36S, 62 56W Salina de Piedras (
Salina de Cagliero) (Buenos Aires Prov.)
40 41S, 62 40W Saline
Pond at the edge of Nat. Route 3, km 1128 (R.
Negro Prov.)approximately 40º 43S, 65º W
Salina del Inglés (Buenos Aires Prov.)

40 43S, 62 28W
5
? MORPHOLOGY? BIOMETRY OF CYSTS AND NAUPLII ?
MORFOMETRY OF ADULTS ? CYTOGENETIC ? INTER AND
INTRAPOPULATIONAL

CROSS-BREEDING
? LIPID PROFILES
6
Lagoon in Comandancia Frías (Chaco Prov.)
24º 30S, 62º 15W Salinas Grandes (NO Córdoba
Prov.) 26º 30-31º 40S, 64º 15-66º
25W Lagoon 40km Northern Los Juríes (Santiago
del Estero Prov.) 28º 03S, 62º 10W La
Antigua Saline (La Rioja Prov.) 29º 54S,
66º 07W Mar Chiquita Lake (Córdoba Prov.)
30º 30- 31º S, 62º 10- 63º 10W
Pampa de las Salinas (San Luis-La Rioja Prov.)
32º S, 66º 41 W Las Tunas Lagoon
(Córdoba Prov.) 33º 44S, 62º 32W Lagoon
in Trenque Lauquen (Buenos Aires Prov.)
35 48S, 62
55W Lagoon in Área de Naicó (La Pampa Prov.)
approximately
36º52S, 64º 24W Salinas Grandes de Hidalgo
(La Pampa Prov.)
37º 13S, 63º 26W Utracán
Lagoon (La Pampa Prov.)
37º 1714S, 64º
3714W Guatraché Lagoon (La Pampa Prov.)

37º4425S, 63º 3315W El Carrizal Lagoon
(La Pampa Prov.)
37º 5048S, 63º 4654W
Colorada Grande Saline or Lagoon (La Pampa
Prov.)
38 18S, 63 42W Colorada Chica Saline or
Lagoon (La Pampa Prov.)
38º 23S, 63º 36W Callaqueo
Saline or Lagoon (La Pampa Prov.)
38º 34S,
63º 32W Salitral de la Vidriera ( de
Garnica) (Buenos Aires Prov.)
38 42S, 62 40W Salinas Chicas
(Buenos Aires Prov.)
38º 44S,
62º 57W Lagoon in San Miguel Field ( of
Winchel) (Buenos Aires Prov.)
approximately 39ºS, 62º 30W Palos Blancos
Saline or Lagoon (Buenos Aires Prov.)
approximately 39ºS, 62º 30W
Lagoon in Hotel Las Termas (Pedro Luro, Buenos
Aires Prov.) 39 30S,
6235W Villalonga Lagoon (de los Gauchos)
(Buenos Aires Prov.)
39 51S, 62 32W Salitral and Saline of
Bajo del Gualicho (Río Negro Prov.)
40º 24S, 65º 13W Salina de
Luzzetti (Buenos Aires Prov.)
approximately 40º 35S, 62º
40W Salina del Algarrobo (Buenos Aires Prov.)

40 36S, 62 56W Salina de Piedras (
Salina de Cagliero) (Buenos Aires Prov.)
40 41S, 62 40W Saline
Pond at the edge of Nat. Route 3, km 1128 (R.
Negro Prov.)approximately 40º 43S, 65º W
Salina del Inglés (Buenos Aires Prov.)

40 43S, 62 28W
7
ARGENTINA
CHILE
Salinas de Cahuil (Pichilemu) 34 25 S
Lagoon in Trenque Lauquen (Buenos Aires) 3548S
8
(No Transcript)
9
MORPHOLOGY OF ADULTS

• The proportions of the body in both sexes
  lengths and widths of thorax and abdomen
• The size of the frontal knob in the antenna of
  the male
• The presence or not of a characteristic
  saddle-like ornamentation on the back of genital
  segments in the female.
• The shape and size of furcal rami and the number
  of setae they bear, in both sexes.

10
Population of Las Tunas Lagoon
11

• 33 44 S latitude
• Assigned to A. franciscana by MORPHOLOGY OF
  ADULTS
• OTHER TECHNIQUES CARRIED OUT
• MORPHOMETRY of ADULTS
• CYTOGENETIC STUDIES
• BIOMETRY OF CYSTS
• INTRA AND INTERPOPULATIONAL CROSSES

12
CYTOGENETIC STUDIES
a) Abnormalities in the meiosis chromosomes
? Heteromorphic bivalents ? Univalents out
of plate at metaphase I ? Laggards at
anaphase I b) Nuclei with an unexpected haploid
number ? Telophase I poles with different
haploid chromosome number ? Meiotic nuclei
with n 22, n 23 and n 21.
13
BIOMETRY OF CYSTS
Mean diameter intermediate among those belonging
to several A. franciscana and A. persimilis
populations.
14
Intra Populational Crosses
15
Intra Populational Crosses
16
Intra Populational Crosses
17
Inter Populational Crosses
18
Inter Populational Crosses
19
Inter Populational Crosses
20
Inter Populational Crosses
21
Viability of the Offspring
excluded cysts improperly shelled excluded dead
nauplii abortive embryos
22
Viability of the Offspring
excluded cysts improperly shelled excluded dead
nauplii abortive embryos
23
Viability of the Offspring
excluded cysts improperly shelled excluded dead
nauplii abortive embryos
24
Viability of the Offspring
excluded cysts improperly shelled excluded dead
nauplii abortive embryos
25
Viability of the Offspring
excluded cysts improperly shelled excluded dead
nauplii abortive embryos
26
On the basis of our results we have hypothesized
that LT population may be subjected at least to a
certain degree of natural hybridization. The
geographical proximity of this population with
other populations of both A. franciscana and A.
persimilis, would allow cysts from both species
to converge sporadically in Las Tunas Lagoon,
brought by means of winds or waterfowls.
27
CYTOGENETICS STUDIES

• Have also been performed in other populations
  assigned to A. franciscana.

• Mar Chiquita Lake
• Salinas Grandes (NW Córdoba Prov.)
• Pampa de las Salinas
• La Antigua Saline

28

• In all these cases, the specific assignation was
  confirmed, as all the individuals have shown

• regular meiosis and n 21.
• nauplii with 2n 42
• all interphase nuclei from somatic cells with
  numerous DAPI and CMA bright chromocenters, both
  in diploid and polyploid cells.

29
Thank you for your attention