Instrumented Nanophysiometer for High Throughput Drug Delivery D. Michael Ackermann1, Jonathan Payne1, Hilary Samples1, James Wells1 Advisors: Franz Baudenbacher, Ph.D.2; Paul King, Ph.D.1 1 Department of Biomedical Engineering, Vanderbilt University,

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Instrumented Nanophysiometer for High Throughput
Drug DeliveryD. Michael Ackermann1, Jonathan
Payne1, Hilary Samples1, James Wells1Advisors
Franz Baudenbacher, Ph.D.2 Paul King, Ph.D.11
Department of Biomedical Engineering, Vanderbilt
University, Nashville, TN2 Department of Living
State Physics, Vanderbilt University, Nashville,
TN

• Design a nano-liter sized nanophysiometer
  suitable for cell culturing
• Implement on-chip peristaltic pumps for low
  volume flow through perfusion
• Incorporate thin film microelectrodes in the
  inflow and outflow channels for monitoring
  physiological parameters
• Develop a LabView user interface to manage valve
  sequencing

• Pump Sequencing ( of valves)
• Cells
• Electrodes

• Use integrated thin film microelectrodes to
  monitor physiological parameters
• pH, glucose, etc.
• Electrodes coated with a substrate specific
  oxidase
• Catalyze reaction producing H2O2
• H2O2 then detected