Supplementary Figure 1

1
Supplementary Figure 1
Red stable co-clustering Blue no co-clustering
2-cluster
3-cluster
5-cluster
4-cluster
2
Supplementary Figure 2
3
Supplementary Figure 3
4
Supplementary Figure 4
5
Supplementary Figure 5
6
Supplementary Figure 6
KRAS mutation
7
Supplementary Figure 7
p0.0001
DUSP4 deletion
p16 deletion
26
10
16
14
5
7
p0.00003
p0.007
17
104
EGFR mutation
n199
8
Supplementary Figure 8
6E-16
Wild type EGFR
EGFR L858R
EGFR del19
5E-16
4E-16
DUSP4 mRNA conc (uM)
3E-16
2E-16
1E-16
0
WT - 2 hrs
WT - 4 hrs
WT - No EGF
WT - 6 hrs
WT - 1 hr
WT - 30 min
del19 - No EGF
del19 - 2 hrs
del19 - 4 hrs
del19 - 6 hrs
del19 - 30 min
L858R - No EGF
L858R - 2 hrs
L858R - 4 hrs
del19 - 1 hr
L858R - 30 min
L858R - 6 hrs
L858R - 1 hr
9
Supplementary Figure 9
A
B
GFP
DUSP4-GFP
DUSP4
Vector
DUSP4-GFP
70KD
GFP
27KD
ACTIN
H358
H1650
H1650
10
Supplementary Figure 10
H1650 cells 24hrs after electroporation
H1650 cells after one week selection
GFP
DUSP4-GFP