Biological Oxidation

1
? ? ?

• ? ? ? ?
• Biological Oxidation

2
???????
????????????????,????????????????????????,????CO2
? H2O????
O2
CO2?H2O
ADPPi
??
ATP
??
3
?????????????

• ??????????????????????,??????????????
• ???????????????????(CO2,H2O)?????????

4
?????????????
????
????

• ?????????
• ???CO2?H2O?????????????????

• ???????????(??,pH????),????????????,??????????????
  ????,??ATP??????
• ????????????????????,??????????????????H2O,??????
  ?CO2?

5
?????????
??CoA
TAC
ADPPi
ATP
CO2
2H
H2O
???
6
??? ??ATP?????The Oxidation System of ATP
Producing

7
?????

• ??
• ???????????(2H)????????????????????,?????????,????
  ?????????(respiratory chain)???????(electron
  transfer chain)?
• ??
• ?????????(2H ? 2H 2e)

8
(?)??????
???????????????(complex)
??????????
?? ? Cyt c ??????????????
9
?????????????????
10
Cytc
???
?
Q
?????
?
?
?
???
11
1. ???? NADH-?????

• ?? ????NADH????? (ubiquinone)

12
NAD?NADP???
RH NAD RH2PO3NADP
13
NAD(NADP)?NADH(NADPH)????
??????????????????????
14
FMN???????,????????????,???????????????FMN ?
15
??????????(Fe-S)???????????,????????Fe2 ? Fe3e
???????
? ?????
16
????
17
??(??Q, CoQ, Q)??????????????????(?CoQ10),????????
????????????
18
???????
19
2. ???? ???-?????

• ?? ????????????

20
? ? ? ?
?????????????????????????,??????????????
21
(No Transcript)
22
(No Transcript)
23
3. ???? ??-????c???

• ???????????????c

24
(No Transcript)
25
4. ???? ????c???

• ??????????c????

??Cyt a3 ?CuB????????????O2?
26
(No Transcript)
27
(?)??????????
??????? ? ???????? ?
????? ? ??????? ? ???????????
28
1. NADH????? NADH ??????Q ??????Cyt c ??????O2 2.
???????? ??? ????? ?Q ??????Cyt c ??????O2
29
NADH?????
FADH2?????
30
(No Transcript)
31
?????
32
???????
?? ????? (oxidative phosphorylation)????????????
???ADP???,??ATP,?????????
??????? (substrate level phosphorylation)
?????????????,?????,?ADP?????ATP????
33
(?)?????????
????????????????? ????????P/O?? ?Gº'-nF?Eº'
34
(No Transcript)
35
??????????
?????????
36
(?) ??????????

• 1. ??????(chemiosmotic hypothesis)
• ?????????,????(H)?????????????????,??????????????
  ????????????????ADP?Pi??ATP?

37
???????????
38
??????
??
39
???????????
???
???
40
2. ATP??
????? F1(a3ß3?de?? )????? F0(a1b2c912??)???
ATP???????
41
?H??????F0?a???c???????,???????,3?ß??????????
ATP???????
42
????????????
(?)???
1. ?????? ??????????????? 2. ???? ??????????????
?????? 3. ???????? ??????ADP?????????? ????
43
?????????????
CO?CN-? N3-?H2S
???A ?????



??? ????A ?????
44
?????????????????
45
?????????(?????????)
???
??? ??
?
Q
???
46

• ???(oligomycin)
• ??????F0??????,??ATP??

???
ATP???????
47
(?)ADP????? ?????(respiratory control ratio,
RCR) (?)????? Na,KATP??????????????? (?)???DNA?
? ????DNA???????
48
???????? ???????
?µH ?????????Hm?????HHc ?????H
??
49
??ATP
?????????????

• ?????
• ??????????21KJ/mol?????,???? ?P?
• ???????
• ???????????

50
(No Transcript)
51
(No Transcript)
52
???????
???????????????????????
53
ATP??????
ATP
???(????) ???(??????) ???(????) ??(???) ??(????)
ADP
??????????????ATP????
54
??????????????
?????????,??????????????????????????(transporter)?
????????
55
????????????

56
(?) ???NADH???

• ???NADH??????????????,?????????????
• ???????
• a-??????
• (a-glycerophosphate shuttle)
• ???-??????
• (malate-asparate shuttle)

57
1. a-????????
58
NADHH
FADH2
FAD
NAD
??? ??
??? ??
??? ??
???
59
2. ???-????????
60
???- ???? ???
NADH H
? ? ? ? ?
NAD
???-a-? ??????
??
??
61
(?) ???????
???????(adenine nucleotide transporter)
??ADP?ATP?????
62
ADP3-
H2PO4-
ATP4-
63
(?)???????????
??????? ? ??????????? ? ???????? ?
?????????????????????? ? ??????? ? ??????
64

• ??? ??????
• The Others Oxidation Enzyme Systems

65
???????????
66
????????????
(?)?????(catalase) ????,????4????
67
(?)?????(perioxidase) ???????,??H2O2????????????

68
?????????
???? ????(O2?)?H2O2?????(OH)????
SOD
2O2? 2H
H2O2 O2
?????
H2O O2
SOD??????? (superoxide dismutase)
69
?????????
3. ????????????
H2O2 (ROOH)
NADP
2G SH
???????
H2O (ROHH2O)
NADPHH
G S S G
??????????????????
70
?????????
(?)????(monoxygenase)
?????
RH NADPH H O2
ROH NADP H2O
??????????(mixed-function oxidase) ????(hydroxylas
e)?
??????????P450 (Cyt P450)???
71
??
72
(?)????
?????????2?????????????2??????
? ?