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??????:?????????????T?????

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Title: ??????:?????????????T?????


1
?????
2
????? ?????
????????????????RNA ???
???????????????????T????? ?????????????
?? ??????
??
???
3
???????
??????????????(10min)
??????????????????????????????(60min)
4
?? ? ??,80-120nm,??????
???(???)????????RNA??? ????????,???,???????
???????DNA???,????????? ?????gag ?pol
?env?? ?????????
5
????????
????????(human immuno-deficiency virus,
HIV)???? ????. ??? ???????????(Acquired
immunodeficiency syndrome, AIDS
),?????80???????????????????,???,????,????????21??
???????????????
6
??,?????????????4000?,????????????,????????
???1985???????????????,??????????????319877?,?
??????102323?,??49845????2009???,?????????????????
??????74?????,??????10.5???
7
1983????????? Montaginer
????1?????????????,??????????????(Lymphadenopathy
Associated Virus,LAS) ?1984???Gallo???????????????
,??????T????????(Human T Cell Lymphotropic Virus
Type ? , HTLV-?) ,??????????????
1986????????????????????? (Human Immunodificidncy
Virus ,HIV) ? ????????????? ?
8
??????? 1.?? ??,??100120nm 2.??
?????????RNA
???????? ??
??????????
??? ???
(???)
??????,??????????
gp41 (????)
gp120 ????? ????,?
???????CD4????????
9
3.HIV???????
3
nef
5
Vif
gag
env
LTR
LTR
pol
vpr
tat rev
HIV????2??????RNA??,??????5???????????????
??9.7Kb,??????????( LTR )?gag?env?pol???????tat?re
v?nef?vif?vpr?vpu???????? gag????????????(P7?
P97)?????(P24)? env?????????????????????????
(gp160), gp160??????????gp120?gp41?
gp120????CD4??????? Gp41??????,???????,???????????
? Pol????????????????????(p66/51),???(p32),??
?(p22)??
10
4. ???? ???????????
?????????T??????T?? ? 5. ???
56?30?????,??????7??0.2?????0.1???,0.3H2O2?0.5
???50??5??????? 6. ??????
HIV???????,?????????? ?????HIV???HIV-1
?HIV-2??,HIV-1???AIDS???, HIV-2????????????

11
7.HIV????????? ??
????????????
??????????
V???? ??gp120
V ?????
???
????
?RNA??????? ? DNA
??
RNADNA? ? ?
? ?
RNA
?????????????? ?? ? ??????
? ?DNA
? ?DNA
??DNA
RNA?H ??RNA
??? ??
12
??? ? ??
??
????RNA
mRNA
mRNA???
??????
??
????????
?????
HIV?????
???????
13
????????? ??? AIDS?????????????????????
???????????????????????????,????????? ????
? ??? ? ?????(???????????????????????????)?
? ???? ????? ??????????,????????CD4
???,??? TH?????????(??????????????????????????????
???)?
14
????CD4???HIV ?????gp120 ????
?????,?????gp120??CD4????,??????????(??????-??T
??? CXCR4)?? ????????,??????gp41,???????????
??,???????? ????????????CCR5??????,?????,?
??HIV?????,??????HIV????????,????????????????,???
??
15
????????(?????????????????????????)
???? (??????????????????)
?????,??????
12??
????
2-4?
??35?????
???????????????????
?????????????
?????
AIDS
HIV????
16
HIV???????? ???????????? ????????????????
????????????????,??????????????????????TCL?????
????????? ????? ??????CD4T??(TH
??)?????????????CD4 ???????(?????????????????????
??????????????????)? ??????????????? ?
17
????????????????,?????????,????????????????
?(?????),??(??????)???(??????)???(?????)??????????
? ?????????Kaposi ?????????
????????,?????????(????)?????,??????????,????????
?????,?????
18
???????? ?????????????????????????
(?)????
??????????????P24
2?
????????????
???????
?? ??
??CPE(?????????????????)
4?
19
(?)?????? ????,???????????
1.??????ELISA????????99,????????????????????????
? 2.??????????(western blot,wB),?????????????
?,?????????HIV?????????????
20
??????(western blot,wB)??
?????(PAGE)??,?????????
??????????????
HIV????
??????,????????
??
?????IgG
?????????
????
21
??????? ??,???????? ???????? ?
??????,?????,??????,???????????????????,????????
????,?????????????????????????
22
??? 1???HIV?????? 2??????HIV?????
3??????????????
23
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24
Crick?????
D N A
D N A
???
??
R N A
R N A
??
? ? ?
? ? ?
25
?? ???? gp120
?????
gp41 P14????(CA) ??? P24????(MA)
??? ???? RNAp7.p9?????(NC) ???
26
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27
HIV ????
28
HIV????
29
(No Transcript)
30
(No Transcript)
31
IL-4
??????
HIV
TH1
IL-2
IL-2
IL-4???
TH CD4
TH2
IL-12
???Tc
???Tc
??C
IL-2.4.5.6.10.TNF.IFN
APC
IL-2.4.5
IgM IgG IgA IgE
IL-2.4.6?
????
IL-5.TNFß
BC
?C
TDAg
IL-4
??C
????
??????????
?????????
32
Chimp research may help AIDS vaccine development
33
(No Transcript)
34
(No Transcript)
35
(No Transcript)
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