Folie 1

1
A
Act, 6h
Act, UV
Act, 3h
con
con
UV
trex1
gapdh
1 0.4 0.1 1 2.8 0.7
IF
C
B
col AP-1
p21 p53
5x
0
50x
10x
5x
0
50x
10x
fold excess
specific shift
ß-Gal activity
7.5J/m2
20 J/m2
control
unbound oligo
MMS
TCDD
D
con 3
0.1 3
0.5 3
con 6
0.1 6
0.5 6
mM nM Time (h)
con 3
1 3
5 3
con 6
1 6
5 6
trex1
gapdh
MNNG
IR
con 3
5 3
25 3
con 6
5 6
25 6
con 3
0.5 3
2 3
con 6
0.5 6
2 6
µM Gy Time (h)
trex1
gapdh
B(a)P
H2O2
con 3
2.5 3
10 3
con 6
2.5 6
10 6
µM mM Time (h)
con 3
0.25 3
1 3
con 6
0.25 6
1 6
trex1
gapdh
Supplement 1
2
mTrex1 Promoter CTGAGGGCCTGAGCATCCAGCCACAAGGTGGCGC
CAGCTCCCACCGCCGTCTTTGCCATATCCACTGATTAAAACTGCACTTCC
TGCTGAGAACACAAGCTTTGTTTCCTGAGTCCGATTTGTGTGGCTGAGAG
GGAGGGACAGGGCAGACCAAGAATTGATGAGATGACTCCTGCTGGAGTTG
AGATGGCCCCCATGGTAACTGATCTGCCCTGAGGAAAAACCCTGCCCCTC
TTCGGGAATTACCCTGAGTCATAGCTTTGGGCGGGGGCCGTGGTGGGGGC
AGGGGTACGTTTGTGGCCAAGGAGGCAGCCCTTTTTAGGAAGGCCTGGAT
CAAGGACTGGGGGAAAGACAAGAAAAGGCAGAGGCTCACGGGCACATTTG
AATCCTAGAGACAACCCAGTTTGAGTTGTCAGTGTTGGGGCAGTAGTCCA
TTTTACCAGCCAGCAGGATCACAGATATGGATCTAGGAAGGCCTTTGGGG
TGCTTGGGGAGGGAGGGAGGAGCGTCAGCACGAGGGCCAGGCCTGGCAGG
CTAACAGCAGGGCGGGCCCTGTTCAGGAGGCAGGCCTATGGGTGGACCCA
GGCAGTTTTACTTCCTCAGCCACACTGCTGCCAGCAGCAGACCCAGCGGA
GTGCCTTGCTGCCTGCTTCTCGGTAAGTGTGGAGTAAGGGTATGGGGTAA
GGCAGAAGAGAATAGGGTTCCCTCACCCTGAAGGTAGTCAGCACTAGGGG
AAGGGCCTCCCTCAAAAGGCAAATAAGTAGTGGATCCATCCCACTAGAAC
AACCCTGCCCTCCAGCTTCTAGTGGCCCTGGATAGTACCTGCTGAAGGGA
AGGGCTGAGCTGCCTTTCTGCACCCCACCTCAGCCCACTTGAGACCTTCC
TGCTGCTACTCATTACCCCATCTCCTCCCCAGGCTCAGCATGGGCTCACA
GACCCTGC GR AGAACA AGTACC WT
GTGTGGCT GAGGGAGGG GCGGGGGCC p53
TGAGATGGCC NF-AT GGAAAAAC SP1 CCCTGCCCC GGGGC
AGGGG GGGCGG NF?B TCCCCA TGGGGA AP1 CTGAGTCA
TACTCA Ref1 TGAGAC RXR TGCCCT TCACCC GGGTGG
AGGCCT c-Maf TCAGCA underlined primer used for
cloning
Supplement 2
3
A
Act-D UV
Act-D
con
UV
trex1
gapdh
B
col AP-1
htrex1 AP-1
col AP-1
htrex1 AP-1
0 4 8 16 0 4 8 16
time(h)
0 4 8 16 0 4 8 16
UV
B(a)P
Supplement 3
4
hTrex1 5 CTGGCCTTGTTTCCTGAGTCTGATCTGTTTGGC
GGAGTGGGAGGGGTGGAGCAGGACCCG mtrex1 96
CAAGCTTTGTTTCCTGAGTCCGATTTGTGTGGCTGAGAGGGAGGGA----
-CAGGG--CA
hTrex1
65 GACCCTGAGTGGCTGGGATCCTTCTTCCTGTCCCTGGCTGTT
GCTGAGCCCGTCCCCATG mtrex1 149
GACCAAGAATTGATGAGAT--GACT-CCTG---CTGG-AGTTGAGATGGC
---CCCCATG
hTrex1
125 GTAACTGATCTGCCTTGAGGAAGGAGCCCTGCCCTGCCTGTGG
AATTGTCCTGAGTCATT mtrex1 199
GTAACTGATCTGCCCTGAGGAAA-AACCCTGCCCCTCTTCGGGAATTACC
CTGAGTCATA

hTrex1 185 GCTTTGGGCTGGGGCCATGGGAAGAAACCAT
TGTGTGGCAGGG--AAGGAGGTGGCTCTT mtrex1 258
GCTTTGGGCGGGGGCCGTGG-----------TG-GGGGCAGGGGTACGTT
TGTGGC-CAA
hTrex1
243 GGCC-CAGGCCTAAACCAGGAAAGCCTGGGAAACTGGGACCCA
CAGGTGGGCATGAAAGG mtrex1 305
GGAGGCAGCCCTTTTT-AGGAAGGCCTGG--ATCAAGGACTGGGGGAAAG
ACAAGAAAAG

hTrex1 302 GCCGCAGCAGGGGCTCCCAGCAGTGTGTAA
GACCGGGAGCTGGTCTGGCACCACTGCCCT mtrex1 362
GC------AGAGGCTCACGGGCACATTTGAATCCTAGAG-----------
--ACAACCCA
hTrex1
362 GGTCCTTCCAGCTGCCTGTCACTGGTATGATGGCCCCGGTGCA
TTGTGCCA-CCAGCAGG mtrex1 403
G----TTTGAG-T---TGTCA---GTGTTGGGGCAGTAGTCCATTTTACC
AGCCAGCAGG
hTrex1
421 C-CACAGCTGTGGATCTTGGAAGGCCTCTGGGGTCCCCCGGGA
GCAGGGGAGTGGGTGTG mtrex1 452
ATCACAGATATGGATCTAGGAAGGCCTTTGGGGT---------GCTTGGG
-GAGGGAG--

hTrex1 480 GGGGGGAACGGATGGTGGTGAGAGGGACAGA
CCAGGCAGGCTGACGAGCAGGGCGGGCCT mtrex1 500
-GGAGGAGCGTCAGCA----CGAGGGCCAGGCCTGGCAGGCTAAC-AGCA
GGGCGGGCCC
hTrex1
540 GGCTCACGTGG---GCCTGTAGGCGGGCCCACGCCAAGTTTC
ACTTCC-C-GCCAC--TG mtrex1 554
TGTTCAGGAGGCAGGCCTATGGGTGGACCCAGGC--AGTTTTACTTCCTC
AGCCACACTG
hTrex1
593 CTGCCAGCGAGAGCCGCGGGAGAGTGTGCAGCCGAGTCACTA
CTGCCTGCCTGCCTGCCT mtrex1 612
CTGCCAGCAGCAGAC-C-----------CAGCGGAGT-------GCCTTG
CTGCCTGC-T
hTrex1
653 GCTACGGTGAGTGTGGCCCCCACAATGGG-ATGGCGCAGGGCA
GGAGGGCCATGGGTTCC mtrex1 652
TCT-CGGTAAGTGTGG-----AGTAAGGGTATGGGGTAAGGCAGAAGAGA
ATAGGGTTCC
hTrex1
712 CCCACCCCAGACTAAGGGGGCACTAGGGGAGGGGCCGAGTCA
TGTGAAGAGGGAGACCCT mtrex1 706
CTCACCCTGAAGGTAGTCAGCACTAGGGGAAGGGCC---TCCC-TCAAAA
GGCAAA----
hTrex1
772 CTCAGACAGTCGAATGTGCTGGTCCCACTAAGGAAACCAC
CTCACCCTCTCCAACTTCCT mtrex1 758
-TAAGT-AGTGGA---TCCA--TCCCACTA---GAACAACC-CTGCC-CT
CCAGCTTCTA
hTrex1
832 G----CCTGAAAA mtrex1 806
GTGGCCCTGGATA
Supplement 4
5
anti-Trex1
TO-PRO-3
overlay
sc8-con
wt-con
wt-con
Supplement 5
6
A
C




Cells ()
Viability (WST, )
UV (J/m2)
12h
24h
B
con
UV
nuclear localization ( of cells)
8?3.1
22?4.7
Supplement 6