You plan to identify this gene in mouse using

1
Quiz3 LC710 11/09/11 name____________
Q1(8 pts) You have just purified this protein
from the eyes of the fish Fugu
MNGTYERATLSMLWAAYMFLLIML
STOP
You make an antibody to this protein and find
that it is only found in the eyes of fish. You
look for that protein in mouse tissues and again
it is only found in the eye.
1 2
1 2
1 2
1 2
You plan to identify this gene in mouse using
(2pts) _______________________as a PCR template.
Design 5 to 3 oligos that will give you
50 or better chance at identifying this coding
sequence in the mouse. You are fearful of
getting to much garbage from your amplification
Codon Probability In fish 1 In mouse 2 organisms
(1pt)
(1pt)
Oligo2_______________________________
Oligo1_______________________________
(4pt) How will you know that Your PCR succeeded
in amplifying the correct fragment (be specific)?
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_____________________________________
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