Methods%20in%20Enzymology%20????(??)

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Methods in Enzymology????(??)
Aily Qin
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1. ????,????

• ???1955?,?????60?????
• ?1 - Preparation and Assay of
  Enzymes(???????),?????????????????
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  ??????
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• ??50,000????????,?????????????
• ?ScienceDirect?????????????

4
2. ?????

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3. ?????

• ???????????????,??????????????

• DNA,RNA,???,???
• ???(????????????)
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• G??????,????????????
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4. ????????????

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• ???,????????????,RNA??,????,????
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5. ????????????

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• ?2015?1?1??????,??????7??
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??????????
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???Volume 58 - Cell Culture ????
Culture cells in vitro simulating in vivo
environment
cell culture medium
cell culture incubator
Cell culture dish
Sterile work table
Pipette
Microscope
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???Volume 58 - Cell Culture ????
applications
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???Volume 58 - Cell Culture ????

• ?????????,?????????????????????????

Part 1
Part 2
Part 3
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???Volume 101 - Recombinant DNA ??DNA
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???Volume 101 - Recombinant DNA ??DNA
GMO

• genetically-modified organism

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???Volume 101 - Recombinant DNA ??DNA

• ??DNA???????????????????????????????,???????????,?
  ?,?????????,??????
• ????????????????(GMO)??????????DNA??
• ???????DNA????????DNA??,??,??,??,???????????????
  ???????,??????????????????
• Volume100,101,153,154,155-Recombinant DNA
  ??????DNA????
• ??Volume 101????????????Sidney
  Brenner(2002?????
• ??/???)? Andrew Fire(2006???????/???)?
• ????????20????DNA???,???????????????????????

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???Volume 152 - Guide to Molecular Cloning
Techniques ????
16
???Volume 152 - Guide to Molecular Cloning
Techniques ????

• ????DNA?????,????????????????????????,????????????
  ???,?????????????,????????????????????????
• ??????????????,????????????
• ?????DNA?????????,???????????,?????????
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• ????????????????????,?????????????????????????????
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???Volume 182 - Guide to Protein Purification
???????
Purified protein
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???Volume 182 - Guide to Protein Purification
???????

• DNA??????????????,???????????????????????????????,
  ???????????????????????,??????????????????,???????
  ????????????????
• 2010?,?Richard Burgess????Murray
  Deutcher????,?????????????????????????

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• ?E.coli?????????????????
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????????????
Volume 182
???????????????
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???Volume 207 - Ion Channels ????

• ?????????????????,??????????????,????????????,????
  ?????????????????????????????????????

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???Volume 207 - Ion Channels ????

• ??????Erwin Neher(1991???????/???),?????????????
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• ????????????

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???Stem Cells ???

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???Stem Cells ???

• ??2003??????Volume 365-Differentiation of
  Embryonic Stem Cells ????????????????????????
• 2006?-2007????????????
• Volume 418-Embryonic Stem Cells ?????
• Volume 419-Adult Stem Cells ?????
• Volume 420-Stem Cell Tools and Other
  Experimental Protocols
• 
  ?????????????
• ???????????review???????????,????????????????????
• ???????????????????,?????????????,????????????????
  ?????????????

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????????????????

• ???????????????????????????
• Volume 529-Laboratory Methods in
  Enzymology DNA
• Volume 530- Laboratory Methods in
  Enzymology RNA
• Volume 533- Laboratory Methods in
  Enzymology Cell, Lipid and Carbohydrate
• Volume 536539541-Laboratory Methods in
  Enzymology Protein
• Volume 537538 - Methods of Adipose Tissue
  Biology
• .

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????ScienceDirect ??www.sciencedirect.com

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• ???????????????20-25???

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?2000?????????
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?2000?????????

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?2000?????????
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