I. PCR- Polymerase Chain Reaction

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I. PCR- Polymerase Chain Reaction A. A method
to amplify a specific piece of DNA.
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B. What is needed?
1.Template A starting piece of DNA. 2. Primers
Tells DNA where to begin the extension.
(PRIMER MIX) 3. DNA Polymerase Enzyme that
allows the addition of nucleotides and can stand
the heat of the thermocycler. (TAQ) 4. Master
Mix Contains the A, T, G, Cs (dNTPs) needed for
the extension process and buffer. 5.
Thermocycler AKA PCR machine. This machine
heats and cools DNA.

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• II. Steps to PCR
• 1. Denature Heat pulls apart the 2 strands of
  DNA
• 2. Anneal The primers bind
• 3. Extension The A, T, G, Cs add
• PCR product
• Round 1 2 strands
• Round 2 4 strands
• Round 3 8 strands
• Round 4 16 strands
• We do 45 rounds!