Bio 328 Immunology

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Bio 328 Immunology Immunoglobulins structure and
Function
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Albumin
a globulin
b globulin
g globulin
Origin
-
Inflammation
Normal
Normal
Myeloma
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Arne Tiselius and Elvin Kabat 1939.
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2014)
1972 Noble Prize in Medicine.
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Nisonoff et al.
Porter et al.
Edelman et al.
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Goat anti-Human Fab binds to both L- and H-chain
goat antiHuman Fc binds only to H-chain.
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Multiple myeloma Bence-Jones proteins Sequencin
g the L-chain 60 kappa 40 lambda (humans) 95
kappa 5 lambda (mice)
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Angiotensin ll
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Van der Waals surface contact between angiotensin
ll and Fab fragment.
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Flu virus antigen and antibody
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Fab and Influenza virus antigen
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Anti-HIV protease Fab peptide
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Fab different anti-hen egg lysozyme
Fab different small epitopes
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Unbound
Ag-bound
Induced-fit
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Unbound
Ag-bound
Anti-hemagglutinin Fab and peptide.
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After
Before
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• Antibody Mediated Effector Functions
• Ab-medicated clearance of pathogens
• Neutralization
• Opsonization
• Complement activation
• ADCC
• Ab isotypes or classes mediate different
  functions
• Fc Receptors

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• How do Antibodies kill Cancer Cells?
• Binding to cell surface receptors and preventing
  their activation.
• Interference with vascularization.
• Induction of apoptosis.
• Induction of ADCC.
• Induction of Complement fixation.
• Delivery of toxins.

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IgG, IgA, IgD IgM, IgE
CH1/CH1 CH1/CH1
Hinge region CH2/CH2
CH2/CH2 CH3/CH3
CH3/CH3 CH4/CH4
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Secretory Component
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1921 K. Prausnitz and H. Kustner 1966 K and Y
Ishizaka
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Immunoreceptor Tyrosine Activation Motifs
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Brutons tyrosine kinase
B-cell Linker Protein
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X-linked agammaglobulinemia
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The End