DYSTROPHIN

1
Gene Sizes Vary
DYSTROPHIN
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Multiple Small Exons are Typical
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4
Full-length first strand cDNA
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Normalize
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Normalize
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Alternative Splicing
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5 RACE
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Short Splicing Signals
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Finding Splice Sites
C T G G T A A
G T
Exon
Intron
15
100nt
INTRON
1st EXON
1.5kb
800nt
5kb
150nt
150nt
Last EXON
Mid EXON
800nt
16
Short Polyadenylation signals
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RNA Editing
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MicroRNAs
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Northern RNA blot
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TaqMan
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OLIGOS
cDNAs
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Sensitivity
25
RNASeq