Time (min)

1
6
A
4
L-methionine uptake (nmol/mg dry weight)
2
0
2
B
1.5
D-methionine uptake (nmol/mg dry weight)
1
0.5
0
0
2
4
6
8
10
Time (min)
Figure S3. Inhibition of the uptake of
1-14CL-methionine (A) and
1-14C D-methionine (B) by the energy
poisoning compounds carbonyl cyanide
4- trifluoromethoxyphenylhydrazone (FCCP) and
sodium arsenate. The wild type cells,
prepared as described in Figure 2, were incubated
with 2 ?M FCCP or 5 mM sodium arsenate at 37 oC
for 10 min prior to addition of 14Cmethionine.
Transport assays were conducted as described in
Figure 2. ?, cells with no energy poisoning
compound ?, cells plus FCCP ?, cells plus
sodium arsenate.