NK??

1
???
????
2
??????????

• ??/????
• ???
• ????
• NK??
• ?????
• T????
• B????

3
??????????
??????????? ????????????
4
?????????
1??????? 2???????? 3?????????? 4??????????
5
????????????
??????
???? ?????
?????
?????
??? ??? ???? ????? ???? ????? ??? T??
NK??
6
??????
??/???? ??? ????
7
??-?????????
GM-CSF?M-CSF
IL-3
?????
?????
???? (??)
???? (??)
8
???????
T???? B???? NK??
9
T ????
10
T???????

• ???????? ?T?? ?
• T?? ????
  ???
• T??

????
???? ????
??????
11
T???????????

• ????(DN) ?????CD4-?CD8-
• ???TCR?CD3,??????????
• ????(DP) ?????CD4CD8
• ???????TCR???????,??TCR????,???????????
• ????(SP) ?????CD4?CD8
• DP???????????,????????,
• ????????????T??

12
?????????
T
????
????
????
CD4??
CD8??
13
T???????????

• ????
• ????

14
T?????????
?DP(????T??)???TCR???????????MHC-I?MHC-II?
????????,???????,?????T??????????MHC????
15
DC
DC
DC
DC
DC
DC
16
DC
DC
DC
DC
DC
DC
17
T?????????
??????????????????????MHC-I?MHC-II??,?????
??????,DP???????????-MHC???,?????,?????????T??????
??????????
18
(No Transcript)
19
T???????
20
B ????
21
B ???????
1?B?????????? ??????(??????)
??????(?????) 2?B???????
????(??????) ????(??????)
22
B?????????
??? ??B ??B ?B
???B ??B ?? ???? D-J?? V-DJ?? ????
?? ???? ???? ???? V-J??
???? SmIg ?? ?? ??
?? IgM IgM/IgD
23
??
??B??
???
??????
24
??B??
??????
25
B???????????

• ????
• ????

26
B?????????
?B???????B????????,??BCR(mIgM)???????,????
BCR(mIgM)?????????????????,?????????,????????,??B?
??????
27
???B??
????
??????
28
B?????????
???B??????????????,??????????,?????B?????
????,?????B???BCR??????,?B?????CD40??,???????CD40?
??Th????,?B??????,???????
29
???????
??????
??
??B??
???
??????
30
NK ???????
?NK?? ?NK?? ???NK
??NK CD34/CD34- CD122
CD122 CD16/CD16-
CD122- CD161- CD161
CD56
31
?????????
??????? ???DC??????? ????????
???DC???DC
?????
32
?????????(?????)
IL-3
lDC
LC
CD1CD14-pDC
lDC
pLDC
?????
mDC
CD34???
MF
Mo
M-CSF
mDC
?????
pMDC
GM-CSF?TNF-a
33
?????????
Lineage-Negative Cells,LNC ????????????? ???????
???????
34
??????????
1?????????? 2?????
35
????????
???????? ???????? ????????
36
??????
1??dT?? 2?NKT?? 3?B1?? 4?NK??
37
?dT ?????

• CD4-CD8-DN T??
• CD8SP T??

38
?dT ????????

• ????????
• Vd2 T???????(HMB-PP)
• ???????(?????????????????????????????????
  ??)
• Vd2- T C1????????
• ??????????????

39
?dT ?????????????

• ???????APC??????
• ??????MHC???
• ?????????????

40
NKT ??
?????? ?????
41
NKT ????????
42
NKT ????????
1??????(?????CD1????????????) 2?????
43
B1 ??
?????? ?????
44
B1 ??????

• CD5
• mLgM?????????

45
B1 ????????

• ????????
• (?????????)
• ?????????(TI-2??)
• ???????IgM???????
• ??????????

46
NK ??
?????? ?????
47
NK ??????

• CD3-CD56CD16

48
NK ????
????????(KAR) ????????(KIR)
49
NK ??????
??NK??CD16?CD2?CD226?CD96?
CD223?CD224?CD69 ???????CD31?CD57?CD15
s?
CD162?CD44 ???? CD56
50
NK ??????????
??? ??? ????
51
?????????
52
?????????????????
53
??/????
ADCC
B
TNF-a
NK
IFN-?
?????
Th1
IFN-?
IFN-?
Th2
MF
NK ??????????
54
??????(APC)
???????????,????????????
???????,???? MHC I?II???,?
???T???????? ?????? ?? 1???APC(professional
APC) B????????????? 2????APC(non-professiona
l APC) ??????????????
55
?????
?????(??)
56
mDC????
???DC
??DC FcR
CR

MR
MHC
II???
LFA-1
ICAM-1

CD80(B7)
CD45RO

57
DC????????
???? ??????? ???? ??T?B??????????
58
??-????
59
??-????
?????????
???? ??
?? CD206 ?????(MR)
??? CD14 LPS??
LPS???? TLR?? Toll???
LPS-CD14??? SR??
????? LDL??????

???????
PS-R
????????
?????? ????V ?3 ??????
????
60
??-????
???????????
FcR CD64?CD32?CD16 CR CD11b/CD18?CD11c/CD18
MHC??MHC?
61
??-??????????
?????? ??????? ??????
?????? ??????? APC
???????????? ????
62
??????
1?????? 2??????? 3??????? 4?????
63
?????????
??T???? ??B????
64
T ????
1?T???????? 2?T??????????????
65
T ??????
TCR-CD3?? TCR????CD4/CD8 ?????
CD28?CD154?CD2 ?????CD152 ?????
MHC?????????(CD95) ??????????????
66
TCRCD3???
TCR
TCR
?
?
d
?
?
?
?
?
?
?
?
?
T???
T???
?
ITAM
?
?
?
CD3??
CD3??
67
CD4 CD8??
T??
TCR
TCR
CD4
CD8
MHC??
MHC??
??????
68
CD28??
TCR
T??
CD28
CD4
CD80(B7)
??????
MHC??
69
CD154??
TCR
T??
CD154 (CD40)
CD40
B??
MHC??
70
CD2??
??????T?????????,?????????,?????????? ??E
-???? ??????T?????
T
CD2??
71
?????
TCR
ITIM
T??
CD152
CD4
CD80
??????
MHC??
72
MHC I
CD80
CD48
CD54
CD8
LFA-1 (CD11a/CD18)
CD2
CD28
TCR
?????????
73
T??????????????
CD4/CD8T?? Th?Tc?Tr ????????T??
74
CD4/CD8T??
CD4T??Th?Tr CD8T??Tc
75
???T??(Th)
Th1? Th2? Th17
76
Th?????
77

Th1
Th2 ??????? IL-2

IL-4
IL-5

IL-10
IFN-?

TNF-?
????????
IL-2 IL-2/IL-4 ????
????
???? ???? Th2
Th1
78
IL-2
79
????T??(Tc)

• ???????
• ???????????????
• ??????????????
• ????FasL??Fas??????

80
Tc????????
81
????????T??
??T??(Tn) ??T??(Te) ??T??(Tm)
82
B ????
1?B???????? 2?B??????????????
83
B ??????
BCR-CD79a/b?? ?????
CD19/CD21/CD81/CD225 ?????CD80/86?CD40 ????(FcR)
CD32?CD23 ????(CR)CD35?CD21 ?????
MHC??????????
84
BCRCD79a/b???
SmIg
CD79a CD79b
CD79a CD79b
ITAM
85
??????
86
CD40 CD80??
TCR
CD28
T??
CD154
CD4
CD40
CD80
B??
MHC??
87
B??????????????
B1?B2 ????????B??
88
B1 ? B2
B1???????? B2?????????
89
B1???B2????
B1
B2 ????? SmIgM
SmIgD

CD5
CD23

?????? IgM?IgG3
IgG1
IgG2
/
/ T????
???????

90
B1???B2?????????
B1
B2 ?????
?????
????

???? ??????? ???????
91
????????B??
??B?? ??? ??B??
92
??B??
??B??
????
?????
?????
???B???????
93

????
1?????????????? 2?????????????????? 3???????????
????????