M r. Wiggles

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M r. Wiggles
38166-06(9/27/06)
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Celebrity Snake- Mr. Wiggles , the travelling
snake

• Arrowhead education center -Okefenokee Joe
• black rat snake Elaphe obsoleta obsoleta
• http//www.okefenokeejoe.com/
• http//www.youtube.com/watch?v_U2knmkrKA0
  Okefenokee Joe sings kin to the wind

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History

• 2 masses The masses first appeared in August of
  2006 and grew very rapidly. .
• Ventral mandible
• Dorsolateral to the eye
• DX Abscess, granuloma, lymphoma/neoplasia
• Surgical excision
• Histopathology and culture

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GMS
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Morphologic diagnosis

• Subcutaneous tissue, Granulomas, Multifocal to
  coalescing, with Intralesional hyphae and
  arthroconidiating hyphae

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Aerobic Culture

• Pantoea agglomorans
• Enterococcus fecalis
• Staphylococcus intermedius

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Fungal protocol

• Sabourauds Dextrose agar
• Mycobiotic agar
• Incubate at 28-30oC for four weeks
• Examine twice a week for growth

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MYCOLOGY MAZE OR-
MYCOLOGY MESS
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• Macroscopic
• Color wide range
• Size wide range
• Texture wide range
• Microscopic- LPCB
• Inconsistency in use of terms to describe the
  microscopic morphology

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Fungal Culture
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Fungal culture
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Further Characterization

• Isolate forwarded to The University of Texas
  Health Science Center at San Antonio for further
  morphological Characterization
• Chrysosporium anamorph of Nannizziopsis Vriesii

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Pleomorphism in fungus

• independently populating organism with different
  morphology Same Biochemical and physiological
  properties
• Anamorph asexually propagating thallus
• Teleomorph Sexuallly propagating thallus
• Total organism Holomorph

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Multiple personality disorder in fungus!
Synanamorphs

• Independently propagating anamorphs
• Pseudallescheria boydii
• Graphium eumorphum
• Scedosporium apiospermum

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DNA Sequencing

• No Matches

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Characterization by Dr Joseph Guarro,
Universitat Rovira i Virigili, Reus Spain

• Morphologic charecterization
• Molecular charecterization

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New fungal species

• Chrysosporium ophidiicola

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• Thanks to
• VDIL, Bacteriology and Histopathology section
• Dr. Deanna Sutton
• Dr. Melissa Vanmeter
• Professor Guarro

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• Questions?