BRUKER Optik GmbH, Ettlingen

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BRUKER Optik GmbH, Ettlingen
HYPERION Imaging Examples for Dr. Bengt Nelander
Nikola Johner Nikola.johner_at_brukeroptics.de 07243
504 658
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Page 3-4 skin tissue Page 5 polymer
blend Page 6-16 multilayer / advantage of the
use of Ge-ATR- objective to improve the spatial
resolution Page 17-19 tissue sample / pixel
binning Page 20 measurement grid / advantage of
the 36x objective versus the 15x objective to
improve the spatial resolution
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For each detector element a complete spectra is
obtained!!!
Visual image of the tissue
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Lipide (? 3000-2800)
Visual image
Proteine (? 1720-1480)
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HYPERION 3000 Polymerblend PTFE/PA
Distribution of PTFE in PTFE/PA Blend in
µm-region
A. Gupper, P. Wilhelm, S. Kazarian Applied
Spectroscopy Vol. 56, Number 12, 2002
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Section of the visual image 3 µm layer
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PET
3µm layer
PE
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Section opf visual image 15x-Objektiv
µm
µm
PE
EVA
3µm layer
PET
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Integration mode B 1328-1271cm-1
PE
EVA
PET
3µm layer
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EVA
PE
3µm layer
PET
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3 µm layer (PET and PE substracted)
Library search, Hummel Polymer poly
(diethyleneglycol isophthalate)
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Advantages of Ge-ATR-Imaging 1) Due to the high
refractive index of Ge (n4) and the
magnification (20x) the spatial resolution is
improved by the factor of 5.3 ! 2) One single
FPA-Detector-element corresponds to 0.8 x 0.8 µm2
measurement area on the sample! 3) The
penetration dephth is about 1-2 µm. Because of
this there is no overlap with the surrounding
material!
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Measurement area with the 20x Ge-ATR-Objective
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3µm layer
Integration mode B 1328-1271 cm-1
PET
The spatial resolution is impoved by the factor
of 5.3x !
PE
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HYPERION 3000 Ge-ATR-Imaging
PET
Significant improvement for detecting the 3µm
layer! No subtraction needed!
PE
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IR-Imaging Combined with Mapping
Visual image of a tissue section
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HYPERION 3000 Contour plot
CH-Region (? 3000-2800 cm-1) Lipide
Proteine (? 1700-1480 cm-1)
4 µm Resolution 0.8x0.55 mm2 Measurement area
Marker peak(? 1355-1270 cm-1)
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Pixel binning
Improvement of S/N! (and reduction of data amount)
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Measurement conditions Imaging Map 4
images Transmittance mode through glass (glass
as reference) 15x Condenser used Comment
Although the configuration of the 36x objective
in combination with a 15x condenser is not
optimal the spatial resolution could be improved
significantly and the small structures on the
grid (1-2µm) could be clearly enhanced.