96 Well Plates

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Jeff and Zhaoduo 20030311 V1.1
DNA from 96 Well Plates to 384 Well Plates
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96 Well Plates
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A B C DEFGH I J K L M N O P
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Note that this is loaded into the printer
with A01 at the bottom right (from the operator's
point of view). See notes on following pages.
384 well plate
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Print head will cover this area
A
A B C D
1A3 2A3 3A3 4A3
1A1 2A1 3A1 4A1
1A2 2A2 3A2 4A2
B
1B1 2B1 3B1 4B1
1B2 2B2 3B2 4B2
1B3 2B3 3B3 4B3
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2
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Jeff and Zhaoduo 20030311 V1.1
Special plate construction (plate "0")
The chlorplast gene plate was transferred to row
A-D of the 384 well plate, plate 1 of
Jeff/Olivier added clones was transferred to rows
E-H, and plate 2 was transferred to row I-L.
Rows M-P of the 384 well plate(New added clones)
remained empty."
Chloroplast
(Recall that the multi-channel pipettor spans 2
wells on the 384 plate. So transfer from 96 well
to 4 rows (each) of 384 well was carried out as
A01-gt A01, A02-gtA03, etc., then B01-gtA02,
B02-gtA04, etc., and C01-gtB01, etc. etc.)
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A B C DEFGH I J K L M N O P
Chloroplast (A-D)
Jeff 1 (E-H)
Jeff 1
Jeff 2 (I-L)
Empty (M-P)
This plate is printed FIRST (including the empty
wells, which of course leave a set of blanks on
the array!) Then the 7 "normal" Plates, followed
by the one "new" plate.
Jeff 2
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Jeff and Zhaoduo 20030311V1.1
3000 clones Microarray Print Format
ALL FROM THE OPERATOR'S POINT OF VIEW!
16 Pins
p o N M L K J I H G F E D C B A
384 well plate
24 22 20 18 16 14 12 10 9 8 7 6
5 4 3 2 1
Dip direction
Slide
Print direction
Each sample prints two times and has 4 duplicates
in a slide (SEE
CLARIFICATION NEXT PAGES!)
ALL FROM THE OPERATOR'S POINT OF VIEW!
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Jeff and Zhaoduo 20030311 V1.1
Note that this is from the operator's point of
view In the layout programs, the chip is
actually represented with rows as the long
dimension and cols as the short dimension so chip
array location (1,1) in the layout program is
printed by pin 13, and the doubling goes across
cols. So all of this is sort of backwards and
upside down from the print head Array, making the
geometry extremely complex!
Spot format (partial)
Pin 13 prints these 4 (as well as many others)
going down the chip
ALL FROM THE OPERATOR'S POINT OF VIEW!
Top half
Bottom half
First touch Second touch
Third touch Fourth touch
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oooooooooooo
??? DISTANCE BETWEEN PINS IN SPOTS ????????
Robot program is Start at upper rightmost
position on chip. Start at lower rightmost
position on source plate. Repeat until out of
source Repeat 10x Load from
source (load 16 wells, all next to one another).
Print at current position. Move
DOWN one spot on chip. Print (replicate
of above). (Note that this makes 20 down prints
10 genes x 2 replicates) Move FAR LEFT
(and up one spot) to same as start position, but
on bottom half of chip. Print two
replicates, as above. Move LEFT 4 wells
on source plate (wrapping around to the next row
and far right at end). Move to LEFT one
spot on chip.
(Note the width of pin array covers the whole
heigth of the array (sans the x19 DOWN moves))
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Jeff and Zhaoduo 20030311 V1.1
Chip format (overview)
Block 2 20x20 (made by pin number 9)
Block 1 20x20 (made by pin number 13)
Block 7 20x20 (made by pin number 6)
Block 14 20x20 (made by pin number 12)
Block 15 20x20 (made by pin number 13)
Block 18 20x20 (made by pin number 1)
(Bottom half replicates top half, above)
Chip ID can be on the right or left, but
is always on the BOTTOM FRONT of the chip
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