PRODUCTION OF ALKALINE PROTEASE

1
PRODUCTION OF ALKALINE PROTEASE

• P.P.Kanekar,S.S.Nilegaonkar,S.S.Sarnaik

2
Status of industrial enzymes

• Total sale of enzymes 500 x 106
• Applications
• Industries
• Detergent industry 40 - 45
• Food industry 30 - 35
• Textile and paper 4 - 6
• Leather 1 - 2
• Feed 0 - 1
• Other 5 - 10

3
Detergent enzymes

• 1913 Laundry detergent Enzymes Patent by
  German Chemist - Otto Rohm- Pancreatic Enzymes
  in Presoak Detergent - Trypsin and
  Chymotrypsin in pH range of 7- 9.
• 1959 Bacterial Protease - Bio 40
• Novo - Alcalase
• Gist-brocades- Maxatase

4
Detergent enzymes

• Before 1965 enzyme market of few million
  dollars.
• Introduction of detergent protease - increased to
  hundred million dollars.
• Dust free protease formulation solved the problem
  of side effects.
• The consumption of detergent enzyme grown ever
  since.
• Total sale 1000 millions (1990).

5
Industrial Applications of Proteolytic Enzymes

• Market Enzyme Application
• Detergents Alkaline protease laundry
  detergents for
• 
  protein stain removal
• Dairy Fungal protease Cheese making
• Beverages Papain Removal of turbidity in
  beverages
• Leather Proteases Bating of leather,
• 
  dehairing and dewooling
• 
  of skins
• Food processing Several proteases Modification
  of protein
• 
  rich material
• Meat and fish Several proteases Recovery of
  proteins from bones and waste fish
• Baking Neutral proteases Dough conditioning

6
Microbial production of proteaseDevelopment of
process

• Raw material
• Deoiled soybean cake DOSC obtained after
  extraction of oil from soybean, rich in protein,
  presently used as base for cattle feed, exported
• Microbial culture Alkaliphilic bacteria

7
Yield and activity of the enzyme

• Protein content of DOSC 42 g
• Crude enzyme protein obtained 1.7 gl-1
• Yield of crude enzyme based 78
• on substrate protein conc.
• Yield of ammonium sulphate 18.7 gl-1
  precipitate of the enzyme
• Activity of enzyme preparation,
• Units /mg protein 249

8
Characteristics of Enzyme

• Stability
• Temperature 30-80o C.
• Maximum Activity 55o C.
• pH 7-12, Maximum Activity pH 12
• In commercial detergent 95-100
• Detergent activity - Destaining of blood stain
  from cotton fabrics.
• Hydrolytic activities on Casein, Gelatin,
  Haemoglobin, Feathers

9
Industries in which this protease can be used

• Detergent industry
• Preparation of animal feed
• amino acid production from
• proteinaceous wastes
• Silver recovery from photographic films