VBTh4 A GLOBAL TREAYMENT FOR THROAT INFECTION

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VB-Th4A GLOBAL TREAYMENT FOR THROAT INFECTION

• By the world renowned
• VITROBIO research institute

PAEXA Award Alternative Methods to Animal
Experiments 1993
Prince Louis de Polignac Award by the
prestigious French Academy of Sciences 1999
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VB-Th41st specific all in one treatment against
throat infection

• Instant relief (1-2h)
• Antibacterial Anti-septic
• Expectorant
• Anti-viral
• Anti-inflammatory
• Healing
• Natural active ingredients
• No side-effects
• Double patent protection.

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FREQUENCY

• SORE THROAT, STREP THROAT, COMMONN COLD, FLU,
• 15-30 Children Adult
• 4-6 days
• COMMON SYMPTOMS
• - Whitish deposition around the throat,
• - Throat pain, tiredness, cough,
• - Loss of appetite, difficulty in swallowing,
• - Thick drainage from the nose, nasal
  stiffness,
• - Headache, fever, sneezing,

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MAIN CAUSE(S)

• Initial viral infection
• (Adenovirus, herpangina, coxsackie, ..)
• Weakening of immune system
• Bacterial growth around the throat
• (Streptococcus but occasionally mycoplasma,
  Neisseria, ..)

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NORMAL INFECTED THROAT
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EXAMPLE OF ECONOMIC LOSSES IN THE USA

• 75 to 100 million physician are visited annually,
• Physician cost above 7.7 billion per year.
• Americans spend 2.9 billion on over-the-counter
  drugs,
• And 400 million on prescription medicines for
  symptomatic relief,
• Nearly 36 patients who saw a doctor received an
  antibiotic prescription,
• Extra costs 1.1 billion annually for estimated
  41 million antibiotic prescriptions,
• An estimated 180 million school days and 126
  million workdays for parents are lost per year,
• When added to the 150 million workdays missed by
  employees suffering from a cold, the total
  economic impact of cold-related work loss
  exceeds
• 20 billion.

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PHYSIO-PATHOLOGY

• Virus infection spreads through direct or
  indirect contact, infects throat cells, and
  replication cycle begins within 8 to 12 hours,
• Virus attack a few cells, create small lesions,
  bacteria attach on the lesions start
  multiplying,
• Newly liberated virus particles attack new cells,
  bacteria continue multiplying and a whitish layer
  appears on the throat. This causes pain,
  inflammation, cough formation which gets
  thicken,
• Natural defence mechanisms neutralise virus and
  bacteria but it takes a week to recover.

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COMMONLY AVAILABLE TREATMENTS (1)

• Topical antibiotics (ex. Locabiotal, Broncorinol,
  ..)
• Antibiotics applied topically help to reduce
  bacterial load around the throat which takes 2-3
  days,
• 2. Camphor containing inhalations (ex. Vicks)
• Decongestion and slight antiseptic effect.
• 3. Pseudo-ephedrine paracetamol ..(ex.
  Actifed, Sudafed) for symptomatic treatment of
  headache, fever, nasal congestion alone or in
  association with an antihistaminic (ex. Humex),
• 4. Anti-inflammatory drugs alone or in
  association with antipyretic drugs as a
  symptomatic treatment (ex. Rhinadvil)

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COMMONLY AVAILABLE TREATMENTS (2)

• 5. Sinus drainage with physiological saline to
  open the respiratory tract,
• 6. Salt water gargles To create an osmotically
  active gradient over the throat so as to
  stimulate outward exudation of liquid and
  bacterial detachment,
• 7. Topical analgesics and anaesthetics (ex.
  Lozenges to reduce pain),
• 8. Different plant preparations,

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THE FOLLOWING PRODUCTS REPRESENT ABOVE 30 000 000
UNITS SALES PER YEAR IN FRANCE

• VICKS INHALER, VICKS VAPORUB, ACTIFED JOUR ET
  NUIT, ACTIFED RHUME, ACTIVOX, ALGOTROPYL,
  AMYGDORECTOL, ANADVIL RHUME, ARKO-GELULES,
  EUCALYPTUS ARKOGELULES PIN BOURGEONS, ATURGYL,
  BALSOFUMINE mentholée, BALSOFUMINE simple,
  BALSOLENE, BIOCIDAN, BIPHEDRINE AQUEUSE
  BRONCORINOL Rhinites, BRONCORINOL Rhume,
  CEQUINYL, CHRONOTROPHIR, CODETRICINE, CODETRICINE
  Vitamine C, COLLUPRESSINE, DAFLON, DENORAL
  comprimés, DOLI RHUME , DOLI RHUME PRO, DRILL
  Rhinites, EFRYL RHINITE, ERGIX Rhume, FERVEX,
  FRAZOLINE, HEXAPNEUMINE, HEXTRIL, HUMEX FOURNIER
  gélules, HUMEX FOURNIER Rhume, HUMOXAL, INHAZYL,
  LOCABIOTAL, LYSOPAINE, MAXILASE, MAXILASE 3000,
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  SOUFRANE, STERIMAR, STILLARGOL, STREPSIL,
  SUDAFED, SUP-RHINITE, TAMIFLU, TRIAMINIC

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Top 10 OTC products in FRANCE
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Inconveniences of existing drugs

• Mostly symptomatic treatment,
• No anti-viral effects,
• Antibiotics No instant effect,
• Antihistaminic Dizziness, dry mouth, dry eyes
  and nose, constipation, confusion, visual
  troubles, excitation,.
• Anti-inflammatory drugs Only reduce inflammation.

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WHICH IS THE BEST AVAILABLE TREATMENT?

• LUKE-WARM SALT WATER GARGLES!
• Relief Millions of persons in poor countries use
  salt water gargles often get relief within 1
  day.
• Mode of action Salt molecules creates an
  osmotically active film over the throat surface.
  This causes outward exudation of liquid from the
  tissues covered with salt to equilibrate osmotic
  pressure between inner outer surfaces. This
  leads to instant detachment elimination of
  micro-organisms.
• Why salt water gargles are not used? Very few
  persons know this activity, tedious to follow 3-4
  times a day, may cause vomiting.
• Why no pharmaceutical company launched a salt
  gargle product? Salt is a natural product which
  cant be patented!
• What is the disadvantage Bad taste, gargling
  procedure, time required (4-5 min each), no
  hydrating effect, no fluidifying effect, no
  anti-viral effect..

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QUALITIES OF AN IDEAL TREATMENT?

• AN IDEAL TREATMENT SHOULD
• Have instant effects (1-2h),
• Detach bacterial layer from the throat surface,
• Exert antibacterial effect,
• Neutralise free virus particles to stop new cell
  infection,
• Remove detached micro-organisms from the throat
  vicinity,
• Hydrate and soothe throat,
• Drain cough,
• Not enter into the throat tissue,
• Not be toxic when swallowed,
• Be natural, colourless, odourless, with agreeable
  taste.

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VB-TH4

• THIS IS A 15-ml SPRAY CONTAINING FOUR BASIC
  INGREDIENTS PRESENTED AS A MEDICAL DEVICE.
• VB-GYL A patented natural hypertonic solution.
• VB-HY A natural food ingredient with strong
  antiseptic and anti-bacterial properties.
• VB-PCD Specific tannins isolated from a plant
  having strong affinity for viral proteins.
• VB-TEA A specific plant tannin with strong
  topical anti-inflammatory properties.

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1. VB-GYL

• This is an osmotically active, natural, dietary
  plant by-product patented throughout the world by
  VITROBIO for its anti-microbial healing
  properties. This product act by creating an
  osmotically active film over the throat surface
  leading to outward flow of liquid from inside
  towards outside the cells. This cause instant
  detachment of all contaminants from the cell
  surface.
• Used as an excipient (diluent) in many
  pharmaceutical, cosmetic, and dietary
  preparations.
• Cant enter into the cells and acts through its
  physical properties.

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2. Anti-microbial effect - VB-HY

• This is a natural food ingredient, osmotically
  active, known as a strong antiseptic as an
  excellent antibiotic.
• Stimulates liquid secreation from the throat
  tissues acting as a natural anti-microbial
  agent and as an expectorant to fluidify the nose,
• Used as food,
• Cant enter into the cells and acts through its
  physical properties.
• An excellent healing agent.

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3. Anti-viral effect- VB-PCD

• A procyanidine (tannin), large complex molecules
  (mol. wt. between 500-20000) present in the
  plants, containing sufficient hydroxyl carboxyl
  groups to form strong complexes with proteins
  (viral protein) and other macromolecules so as to
  precipitate these molecules.
• Bounded virus particles cant infect new cells.

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Influenza virus does not have the processing
protease for viral membrane fusion glycoprotein.
Therefore, to enter into the cell, virus uses
host cell proteases that proteolytically activate
virus-cell fusion.

• Protease
• Virus

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MODE OF VIRAL CELL INFECTION

• Virus alone cant enter into the cell
• Virus associates with proteases

• 3. Host cell protease activate virus cell fusion
• 4. Intracellular virus replication causes cell
  death, formation of lesion subsequent bacterial
  infection.

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EXISTING OR POSSIBLE ANTIVIRAL DRUGS

• Supply nuriminadase inhibitors but viral
  resistance is a common phenomenon (ex.
  Oseltamivir, Zanamivir, peramivir),
• Up-regulate the production of endogenous protease
  inhibitory compounds,
• Increase mucosal immunity (ex. With Ambroxol)
• Increase IgA level (ex. With the macrolide
  antibiotic Clarithromycin),
• Stop viral penetration or replication into the
  cells,
• Give additional topical protease inhibitors.

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ANTI-VIRAL MODE OF ACTION OF VB-TH4

• Due to strong affinity for viral glyco-proteins,
  VB-TH4 procyanidin I bind neutralise virus
  perticles.

• VB-Th4 procyanidins
• Virus
• Procyanidin-virus binding

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ANTI-PROTEASE MODE OF ACTION OF VB-TH4

• VB-TH4 Procyanidin II binds to proteases
  (Trypsin, elastase, ..). Stopping virus-cell
  fusion further cellular infection.

• VB-Th4 procyanidins
• Proteases
• Procyanidin-Protease binding

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4. Anti-inflammatory effect - VB-TEA

• A naturally occurring tannin with a strong
  proven anti-inflammatory properties.

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WHY PLANT TANNINS?

• Vitrobio isolated, purified tested hundreds of
  plant tannins for specific properties. Some
  tannins binds to specific viruses or exert
  anti-inflammatory effects. Tannins are big
  molecules cant enter the cells. Due to their
  strong binding properties with other molecules,
  they are also used to enhance product viscosity.
  Due to their physical mode of action, tannins can
  be used for external application as a medical
  device.

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MAIN PROPERTIES OF VB-Th4 COMPARED TO THE
AVAILABLE DRUGS

• ANTI-BACTERIAL activity due to osmotic exudation,
• ANTIBIOTIC natural properties of VB-HY
  ingrediant,
• INSTANT (1-2h) relief due to rapid onset of
  osmotic exudation process,
• CLEANING of detached bacteria due to liquid
  generation,
• ANTIVIRAL activity due to virus binding
  procyanidins,
• HUMIDIFYING and expectorant effect due to osmotic
  activity,
• PAIN RELIEF,
• 100 natural,
• No side-effects,
• Ready to sale in Europe due to Medical Device
  status,
• 1st product with all the properties required for
  throat sore treatment.
• Protected by 2 international patents.

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MEDICAL CLASSIFICATION

• Class I or IIa medical device (decision March
  2009)
• For the treatment of sore throat (Claims
  antibacterial, anti-viral , expectorant)
• Complete registration dossiers, proof of activity
  of each ingredient and other confidential
  information are available.
• Contact Dr. Ravi SHRIVASTAVA naturveda_at_wanadoo.fr
  Website vitrobio.com