DEVELOPING FLUORESCENT LIFETIME PROBES USING BODIPY DYES

1
DEVELOPING FLUORESCENT LIFETIME PROBES USING
BODIPY DYES

• Thomas P. Gonnella

2
Probes
3
Targets - Extended
4
WTM
5
Lifetime
6
Lifetime
7
Calculating Average Lifetime
8
Photodecompostion(Blank Wild Mutant)
9
Interconversion
10

• VRN1-sequence 1488 bp
• GATGAGACGCTTGACAACAGTGTATTGATGGATGTCTGGTCGGTATACAC
  GCACAGCACAGTACCCCTACTCTAGGACTGGCGAGTATCTTTCATTCATT
  CCAGAAATACGCGGGTCGGCCAAAAGTAGAAAAATACACTGCGCCCACTC
  AATCCACGCAGCGCACTGCACTGCACAGCAACGCTTCATGTCAAAAGTCG
  AGCTCAAGCATGCACGCGATGGACGCGGCGCGAATGACCCGGGCGGCACG
  ACGCGAGTGCCCGCCGCGCCCGCCCGCCTGCCCCGCAGCCGACCTCTCCC
  AAACGGGACAAGCGAGACGGCCCAAAACGAGCAAGGAAAGCAGCCTCCTA
  CTGTGGCAGCCCGCCCCCACGACCGTCATCTCGCCTTCCATTCCATTTTC
  CCTGGACGGACCAGACCCGTCCGAGCCGCCCTGACCTAGCCAGCCAGCAT
  TTCCTCTTTCGTCCCCCGCCGCCGTGACCAAAAAAGCAAAAAAGGAAAAA
  GGGAAAATGCTAAAGGAAAAAACTCCGCTCTTTCCCTTCTTCTAGGCCTA
  GGGTACAGTAGAATATTATAAAAGGAAAAATTCTGCTCGTTTTTTGCTCT
  GTGGTGTGTGTTTGTGGCGAGAGAAAATGATTTGGGGAAAGCAAAATCGG
  GAGATTCGCACGTACGATCGTTCGACACGTCGACGCCCGGCGGGCCCGTG
  GTGGGGCATCGTGTGGCTGCAGGACCGCGGGGCCCCGCGGGGCGGGCCGG
  GCCAATGGGTGCTCGACAGCGGCTATGCTCCAGACCAGCCCGGTATTGCA
  TACCGCGCTCGGGGCCAGATCCCTTTAAAAACCCCTCCCCCCCTGCCGGA
  ACCCTCGTTTTGGCCTGGCCATCCTCCCTCTCCTCCCCTCTCTTCCACCT
  CACCCAACCACCTGATAGCCATGGCTCCGCCGCCTCGCCTCCGCCTGCGC
  CAGTCGGAGTAGCCGTCGCGGTCTGCGGGTGTTGGAGGGTAGGGGCGTAG
  GGTTGGCCCGGTTCTCGAGCGGAGATGGGGCGCGGGAAGGTGCAGCTGAA
  GCGGATCGAGAACAAGATCAACCGGCAGGTGACCTTCTCCAAGCGCCGCT
  CGGGGCTTCTCAAGAAGGCGCACGAGATCTCCGTGCTCTGCGACGCCGAG
  GTCGGCCTCATCATCTTCTCCACCAAGGGAAAGCTCTACGAGTTCTCCAC
  CGAGTCATGGTAAATTAAGCACGCGCTGTCTTTAAATTTGTTCCCCAATA
  CGCCTTCGATTTCGATTTCCTGCGCACCGTTCTGGTCCTGCGAGACGACC
  CGGCCGACCCCAGGGCCTTCTCCATTTCCGCGCTGCTGTTTGGTTGATTC
  CGTTTGCCCGCTCGCTGTTTCCATCCGATTCTGCCGTGGCTGCTTGCTCG
  TTTTTCTTAGAATCGATGGGGGAGCTGGCGTTCCGCGCGGCCGCGATTTC
  TTGCTATGGGGGGTAGGGCGCGCGATGGGTCGCCGGG