Ribozymes (RNA Enzymes)

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Ribozymes(RNA Enzymes)

• David Peterson

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Enzymes

• Catalyze reactions
• Very specific
• Act on substrates
• Active site

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Enzymes Bind Substrates
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Enzymes Bind Substrates
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Enzymes Bind Substrates
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Structure of RNA and DNA
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Information Flow in Cells The Central Dogma of
Molecular Biology
DNA RNA Protein
Transcription
Translation
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Semiconservative DNA Replication
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Amino acids combine to make peptides and proteins
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mRNA Splicing
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Ribosomes

• Site of protein synthesis
• Made of RNA and protein

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Tetrahymena thermophila
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Self-Splicing RNA
Cech, Zaug, and Grabowski.1981. Cell 27 487-496
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Single-Stranded RNA Can Form Intramolecular Base
Pairs
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Intramolecular H-bonds in the Tetrahymena Ribozyme
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3D Structure of the Self-Splicing Intron
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Ribosomes

• Site of protein synthesis
• Made of RNA and protein

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Nobel Prize in Chemistry 1989
Tom Cech
Sidney Altman