BC basic - from big bang

1
From Genome to Proteome
Genome
Proteome
Juang RH (2004) BCbasics
Systems Biology, Integrated Biology
2
Proteome Reflects Gene Expression Repertoire
Proteolytic digestion
Proteolytic fragments
Pure protein
2D electrophoresis
Sample
Capillary Electrophoresis HPLC
MALDI-TOF
Mass spectrum
LC/MS/MS
Database searching
Amino acid sequencing
Juang RH (2005) BCbasics
3
Identify Unknown Protein by Mass
Unknown protein
Protease digestion
MALDI-TOF
m/z
? Compare peptides and identify the unknown
protein
Juang RH (2004) BCbasics
4
Protein Sequencing by Mass
Unknown protein
Protease digestion
LC
P4
ESI-Mass/Mass
R
V
W
S
? Phosphorylation site
G
K
G
? Direct sequencing
-GKGSWVR
Juang RH (2004) BCbasics