Chapter 3 Metalloproteins and Metalloenzymes

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Chapter 3 Metalloproteins and Metalloenzymes

• 3.1 Uptake??, Storage?? and
• Transport??
• 3.2 Biological Chemistry of Iron, copper,
• and Zinc
• 3.3 Biological Chemistry of Other Metals

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Distribution of Iron, Zinc, and Copper

• Fe out of a total of 4-5 g 60-70 hemoglobin
  7-15 ferritin??? 3-5 myoglobin???? 0.1
  catalase????? 0.1 cytochromes?????
• Zn out of a total of 1.4-2.3 g liver? 55 mg
  kidney? 55 mg muscle?? 54 mg heart 33 mg
  pancreas? 29 mg spleen? 21 mg lung? 15 mg
  brain 14 mg adrenal???12 mg prostate??? 102 mg.
• Cu out of a total of 80-120 mg 8 mg in liver
  15 mg in heart, spleen, kidneys, brain, blood
  concentrated in the pigment parts of eyes

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Protein Carriers in Blood Plasma

• Carrier Ion
• Transferrin???? Fe3
• Ceruloplasmin?????? Cu2
• Albumin??? Zn2, Cu2
• Metallothionein????? Zn2, Cu2, Cd2,
• 
  Hg2, Co2, Pb2,
• 
  Ag
• Phosphoproteins Ca2
• Non required Mg2, Na ,
  K

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Ion Storage

• Proteins Ion
• Ferritin??? Fe3
• Metallothineine Cu2, Zn2,
• Heavy Metals
• Ceruloplasmin Cu2, Zn2
• Serum Albumin Cu2, Zn2
• Phosphoproteins Ca2

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Metalloproteins and Metalloenzymes

• conjugated proteins protein
• prosthetic
  (cofactor)
• Holo-enzyme apo-enzyme
• prosthetic (cofactor)

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Classification of Metalloproteins

• Dioxygen Carriers???
• Redox Protein??????
• Classification of Metalloenzymes
• Oxido-reductase ?????
• Transferase???
• Hydrolase???
• Lyase???
• Isomerase???
• Ligase???

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Bioinorganic Chemistry of Iron

• Oxygen Carriers hemoglobin????, myoglobin????,
  hemerythrin??????
• Redox proteins iron sulfur protein????,
  cytochrome c????c, cytochrome b5????b5, etc.
• Redox enzymes cytochrome p450????p450,
  peroxidase?????, catalase?????,etc.

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Bioinorganic Chemistry of Zinc

• Hydrolysis Enzymes - Lewis Acid
• carbonic anhydrase????
• carboxypeptidase???
• alkaline phosphatase??????
• proteinase
  
• NAD-dependent dehydrogenases
• alcohol dehydrogenase????
• glutamate dehydrogenase??????
• lactate dehydrogenase?????
• malate dehydrogenase??????
• Structure Roles

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Bioinorganic Chemistry of Copper

• Oxygen Carriers
• Hemocyanin????
• Redox Proteins-Electron Transfer Protein
• Plastocyanin????
• Azurin???
• Stellacyanin
• Oxido-Reductase

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Oxido-Reductase Enzymes Superoxide
Dismutase??????? Tyrosinase???? Laccase?? Ascorbat
e Oxidase??????? Galactose Oxidase?????? Dopamine-
B-Hydroxylase?????? Amine Oxidase???? Urate
Oxidase????? Cytochrome c Oxidase????c???
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