Semen Evaluation

1
Semen Evaluation
??.?.??. ???????? ??????? ????????????????????????
?????????????
2
(No Transcript)
3
Semen collection

• Semen collector
• Bull handler
• Teaser handler

4
Semen collection

• Artificial vagina

5
Semen collection

• Electro-ejaculator

6
Semen collection manual manipulation
7
Semen evaluation

• Macroscopic evaluation
• Microscopic evaluation
• Special evaluation

8
Macroscopic evaluation

• Volume Beef bull 5 -10 ml
• Dairy bull 10-15 ml

9
(No Transcript)
10
2. Consistency

• Watery
• Translucent
• Milky
• Creamy
• Glue -like

11
3. Density

• D thin
• DD medium
• DDD thick

12
4. pH

• Beef bull 6.7
• Elephant bull 7.04
• pH meter or pH paper

13
5. Contamination

• Pus
• Blood
• hair
• Urine

14
6.Color

• Transparent gray
• Evident gray
• Strong white , Yellowish white
• Yellow

15
Microscopic Evaluation

• 1. Mass Activity
• 10 X
• 0 NO motility
• ??????? ???????????????
• ?????????? ??????? ?
• ????????? ??????????

16
2. Motility

• 10-40 X with Cover glass
• Progressive movement
• motile sperm

17
(No Transcript)
18
3. Concentration

• Haemocytometer method
• Flow cytometry
• Electronic particle counter
• DNA assay
• Computer-assisted semen analyzers (CASA)
• Turbidity
• - Photolectric colorimeter
  
• - Spectrophotometer

19
(No Transcript)
20
(No Transcript)
21
Haemocytometer method

• Formal saline dilute
• Boar 1 50
• Bull 1 200
• Stallion 1 100- 40 X
• - (N x D x 250 ) / A mm3

22
(No Transcript)
23
(No Transcript)
24
(No Transcript)
25
4. Percentage of lived sperm

• Eosin ,Nigrosin, Fast green analine blue
• 100 x
• 200-500 sperm

26
(No Transcript)
27
(No Transcript)
28
5. Sperm morphology

• Dry preparation ---gt Head of sperm
  Indian ink Eosin-Nigrosin
  Geimsa-stain Wrights stain
  William stain

29
(No Transcript)
30
Sperm morphology

• Wet preparation ---gt tail of sperm
• Formal saline 12 ,15

31
(No Transcript)
32
Classification of Abnormal Sperm

• Primary abnormality ,Secondary abnormality
• Major or Minor sperm abnormality

33
Major sperm abnormality

• Proximal cytoplasmic droplet Pyriform head

34
Double headed sperm
35
Strongly fold ,coiled tail ,coiled around the head

36
Middle piece defects
37
Maldeveloped or undelveloped
38
Diadem defect or Pouch formation
39
(No Transcript)
40
Minor sperm abnormality

• - Distal cytoplasmic droplets

41
Tailess normal heads and detached sperm head
42
Simple bent tail or Terminally coiled tail
43
Narrow ,small ,Giant head
44
Abaxial implantation
45

• Abnormal acrosome

46
Special sperm evaluation

• Metabolic reaction rates
• Glycolysis
• Respiration
• Hydrogen ion concentration
• Penetration hamster ova
• Filtration
• Sexing semen
• etc