Genetic Technology

1
Genetic Technology

• 13.2 Recombinant DNA

2
Genetic Engineering

• Selective breeding is one way to increase allele
  frequency
• Genetic engineering, or recombinant DNA is faster

3
Genetic Engineering

• Genetic engineering cutting DNA from one
  organism and inserting into another
• Recombinant DNA is made by connecting
  fragments from different sources
• Transgenic organisms contain foreign DNA

Tobacco with firefly glow
4
Genetic Engineering

• How is it done?
• Step 1 restriction enzymes cut DNA
• Each enzyme cuts a specific DNA segment

5
Genetic Engineering

• How is it done?
• Step 1 restriction enzymes cut DNA
• Step 2 Vectors transfer DNA
• Vectors virus, bacteria DNA or plasmid
• Vectors carry cut DNA segment into cell

6
Genetic Engineering

• How is it done?
• Step 1 Restriction enzymes cut DNA
• Step 2 Vectors transfer DNA
• Step 3 Gene splicing
• Rejoining of DNA fragments
• DNA is copied when cell replicates

7
Genetic Engineering
8
Cloning

• Clones are genetically identical copies
• Dolly was the first mammal to be cloned from an
  adult cell, 1997
• Cloning helps us understand how cells and genes
  work, may develop cures for diseases

9
Applications of DNA technology

• Sequencing DNA process to read nucleotide
  sequence
• Can help study of specific genes

10
Applications of DNA technology

• Recombinant bacteria in industry
• Break down pollutants
• Recombinant DNA in medicine
• Create hormones, insulin
• Transgenic animals created to have human diseases
  for study