??????????????,???????????????????????,??????????????????:Reference Manager 9.0

1
?????????
2
????????

• ??????????????,???????????????????????,???????????
  ???????Reference Manager 9.0

3
Reference Manager 9.0

• ?????????????PubMed?609?Z39.50?????????,??????????
  ?????
• ??????????????,?????????????????????????????????WO
  RD?????,?????????????????????,????????????????????
  ,????????????????,???????????

4
2. Endnote 3.1.2

• ?????????????,????????,???????????.

5
?? ??????

• ???????,??????????DNA?RNA?????????????,??????
  ??????????????????????(motif)???RNA???????????????
  ??????????????

6
1? ????Omiga 2.0

• Omiga???????????????,????????????????????????????
  ??,???????
• ?Clustal. W????????,??????
• ??????????????(Motif)??????(ORF),?????PCR??????
• ??????????(Proteolytic Sites)??????????
• ??????????????,?????????????
• ??,?????????????????????????????,?????????????????
  ????

7
DNASIS 2.5

• DNASIS for Windows 2.5????????(Hitachi Sofeware
  Engineering Co.,Ltd.)97??????????????????
• ??????????????????,???DNA?RNA????????????,????????
  ???????????,?????????????
• ?DOS??,DNASIS 7???????????????????????????,???????
  ??Win??DNASIS ????????

8
DNATools 5.1

• ?Omiga, DNAsis, PCgene????????????,????????
• DNATools??????????,???????????????????????????????
  ?
• DNATools?????,??????????????????????????????(?????
  ????????),????????????,??????????????DNA??,???????
  ?????

9
DNATools

• ??????DNATools???(DNA???????),???????????,????????
  ??????????(????DNA?????????)???????????????????,??
  ??????????????????????????????????????????????????
  ??????,????????????

10
DNATools

• ????????????,DNATools???????????????5????/????,??
  ???????????,????????????????,?????????????????????
  ??

11
2?????????

• DNAssist 1.0
• ?????????????????,??cNNNNNNNNgaatt?????????EcoR
  I????
• DNAssist 1.0???????EcoR I??????
• ??DNAssist????????,??????????,????DNASIS?????,????
  ??(????,???????)?

12
3? ????

• Primer Premier5.0?????Premier?????????PCR????????
  ????????????,?Plasmid Premier2.02?????????????????
  ?????????????????(Genetank),??????(Primer
  Design),??????(Restriction Sites)?Motif?????

13
Primer Premier5.0

• ????,???????????????
• ?????????????????????????,?????????,??????????????
  ??????????????
• ???????,?????????,?????????????
• ???????

14
4? ???????(Alignment)

• GeneDoc 3.2 GeneDoc????????????????????,??????
  ???,??????????????????,???????????????,??????,????
  ?????

15
MACAW 2.05

• ?????????????(Multiple Alignment Construction
  Analysis Workbench, MACAW)??????????????????????

16
MACAW

• ??????
• 1. ???????????,?????????????
• 2. ???????????????block???????????
• 3. ????????,????????block??????????????
• 4. ???????????block???????????????????????,???????
  ?????????MACAW????????

17
Clustal X

• ?????????????????(multiple sequence
  alignment)????
• ???????????????????,????????,??????,?????????,????
  ????????????,??PCR??,????? ????????????,Clustal
  X???????????

18
5? ????

• Gene Construction Kit 2.0????????????????????????
  ??,????????????????????????,???????????????(????
  ??)????????????????????????????????????,?????????
  ???????????,?????,???????

19
Plasmid Premier2.02

• ??????Premier Biosoft????????????????,??????????,?
  ????????????????????????(Genetank),??????(Plasmid
  Design),??????(Restriction Sites)???????(Motif)???
  ????????????,??????Genbank?Vector?????????????,???
  ??,????????????,?????,???,?????????????????Header?
  ???????????????????

20
6? ???(motif)??

• Primer Premier 5.0??????????????????,????????????
  ??????????????????????????????????????????????

21
7? RNA??????

• RNAdraw?????RNA?????????? 1. ? ?Windows???????
  (multiple document interface) ??,?????????????????
  ?????????????????????????????????????????????????
  ????2. RNAdraw????????????????????????????????????
  ?/???????????? 3. RNA??(RNA Library)??????????????
  ???RNA?????

22
RNAStructure 3.5

• RNA Structure ?????????,?Zuker???RNA??????RNA???
  ?????????????????????????Turner????????????????Zuk
  er?????,??????????RNA?????

23
8? ????????

• ????Antheprot 4.5 ?????????ANTHEPROT
  4.5?????????????????(Institute of Biology and
  Chemistry of Proteins)????????????????????????????
  ?????????????,?????????????,??????,???????????????
  ????????????????????????????,???????????????????

24
9? ??????

• ????????????????????,???????????????,????????????
  ?????????????????????RasMol 2.7.1

25
RasMol 2.7

• ???????????????????????????,????????????,?????????
  ,????Internet??????????,????????????????,????RasMo
  l 2.7??????????,????????????,????????????????,????
  ???????????????????????????

26
RasMol 2.7

• RasMol 2.7????GlaxoWellcome??(?????????)????????R
  oger Sayle? RasMol 2.7??????????,??????,??????,???
  ?????,???????????,?????DNA?RNA,
  ????,?????????????????,???????????????????????????
  ?????????????????????????,????????????????????????
  ??

27
Cn3D

• ??NCBI?????????????????,??????????NCBI????????????
  ??MMDB???????????,??????????????,?????,???????,???
  ??????

28
Cn3D

• ????????,?????????????????,????????Rasmol?Weblabvi
  ew?????????????,??????NCBI????????MMDB?????,??????
  ????????????????Entrez???????????????,?????????

29
Cn3D

• ?Cn3D??????????????????????????????,??????????????
  ??????VAST????????????,Cn3D???????????????Blast??
  ??????Genbank?????????????????,???????????????????
  ?????

30
10? ????

• ???????????????????,????????????????????????????
  ??????Seqverter 1.3

31
Seqverter 1.3

• ????,?????????????????????????????????????????????
  ????,????????????????

32
11? ??????

• ????band leader 3.0 ????DNA????????????????
  ????????????????????????????,???????WINDOWS????
  .BMP,??????????

33
?? ????????

• 1?   ?????????Pov-Ray for Windows 3.1
  ???????,?????pov????,????????????????????????,???
  ??????????????????????????????????????12801024?
  ????molmol 2.5.1?????pdb????????????,??????????

34
????????? Sequin 2.90

• ???????? Sequin 2.90??GenBank?EMBL?DDBJ????????
  ???????????????????????,?????????,????????????????
  ???????,???????????,?e-mail?????

35
Digest

• DIGEST ????DNA?????????????.??????????????,?????
  ?????????WISCONSI.920?,???????????????????.???Don
  Gilbert?????UREADSEQ,???????????????

36
PCMolecule

• PCMolecule ????????????,?????.mcm??????????

37
Pcrare

• PC-Rare??????????????????(OFD)???PCR???,???????
  ??

38
Redsoft Plasmid

• Redasoft Plasmid ??????????????????????????????
  ??,Redasoft Plasmid ???????????????????,?????????
  ?????????????????????web???????????????,??????????
  ????????????????????????????1000???REBASE????????

39
Redasoft Plasmid

• ?????????????????????????????? (What You See Is
  What You Get)?????????????????????????????????????
  ????????????????????????????????????????????????Wi
  ndows????????e-mail?????????

40
TreeView

• Tree View??????????????,?????NEXUS?PHYLIP????????
  ??,?????,????????