UGA ?????? ?????? ??????? selenocysteine ????? ?????? STOP

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UGA ?????? ?????? ??????? selenocysteine ?????
?????? STOP

• ??? ?????? ?????? ?????? ?????? ?-UGA ???????? ??
  "???????"

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Selenocystine -?????? ??????? ?- 21'

• ????? ?????? ????? ???????
  ??? ??????? ????? ???, ????
  ???? ????? ?? ??????? ????????
  
  ???????? ?????-?????.
• ????? ?? Selenocysteine ???? ??? ?? cysteine ??
  ?? ????? selenium ????? ???? ??????? ?????.

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??? ??? ????? ????? ??????
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???? ?????? ??????? Selenocysteine
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translational readthrough ????? ?? ?????? ?-
Selenocysteine

• ????? ?????? ?????????????? ?????? ????? ??????
  ?? ???? ?????? ??????? ????? ?- UGA ????? ????
  ??? ?- stop codon (umber codon).

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?????? ?????? ?? stop codons
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• ????? ????? ?????? ??????? ?????? ????? ??
  ?????? ???? ??????????? ????????????
• ?? ???? ??????
• stem-loop RNA structure
• ??????? ???????.

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sel operon ??????????? ?????? selenocysteine
??????

• ?????? ?????? ?? Selenocysteine ????? ?????? ??
  E. coli ???? 5 ??????? ??????

• ???? (cis-acting mRNA element) ?????
  ????? ?????? ???? ?- 3 ?- UGA ??????
  CECIS (selenocysteine insertion
  sequence).
• 4 ??????? ???????? ?- sel operon.

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sel operon
???? ???
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????? ?????? selenocysteyl-SectRNA )selA)
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?- selenocysteyl-SectRNA ????? ?? ??? ????? ?????
?????? (SelB) ??? ????? ?????? ????? ????? ????
?- mRNA, ?????? ???? UGA ???? ????????? A
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????? ???? ?-"?????-?????" (SECIS)
????? ???? ??????- UGA
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?????? ????? 2 ?????? ??????? ?- SECIS Element
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??????????? ?????? ????? selenocysteine ??????
????? ?? ??? ?? ??? ???? SECIS ????

• ?????? ?? ????? ?? ???
• SECIS binding prorein (SBP2)
• ???? ????? ????? ?????? ???????? (eEFsec)

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??????????? ???????????? ?- SECIS ????? ?- 3
untraslted region


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???? ???????

• ?????
• ??? ?????? ?????? ?? ?????? ???????
  ??????????? ????????????
• ????? ?????? ?????? ??????? ??????? ??
  
• ??????? ?????????? ?????
• ??? ????? ??????? ?? ?????? ???

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???? ??????
?????, ??????? ?????, ?? ????? ??? SECIS ????
selenoprotein ????? ????? ????? ?????? ????.
????? ??? ?- SECIS ???? ?- cDNA ?????? ?????
???????.
????? ???? ??? ?- SECIS ???? ???? ????? ?????
????? ??"? ?????? ??????.
????? ????????? ?? ??? ?- SECIS ???? ????? cDNA
?? ??????? ( selenoprotein) ??????????? ????? .
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??
??

• ????? ????? ??????? ?? ????? ?????.
• ????? ????? ??????? ???? ???? ?-SECIS ?????? ??
  ?? cDNA.

????? ???? ?????? ?? SECIS ?? ??? ?????? ?????
??? ????? ?? ?- cDNA ?? selenoproteins
?????? ?????? ???????? ?? SECIS ???????
??????????? ?????? ????? ??????.
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gtgi6649218gbAF166124.1AF166124 Homo sapiens
selenoprotein X mRNA, complete cds 1
ggcacgaggg gaggtggacg cgggtaccgg cggtcgtcgg
gtcggcagcc tttggtcagt 61 tggcagcggc
aagcgcgctg cggttccggt ggcgccatgt cgttctgcag
cttcttcggg 121 ggcgaggttt tccagaatca
ctttgaacct ggcgtttacg tgtgtgccaa gtgtggctat
181 gagctgttct ccagccgctc gaagtatgca cactcgtctc
catggccggc gttcaccgag 241 accattcacg
ccgacagcgt ggccaagcgt ccggagcaca atagatctga
agccttgaag 301 gtgtcctgtg gcaagtgtgg
caatgggttg ggccacgagt tcctgaacga cggccccaag
361 ccggggcagt cccgattctg aatattcagc agctcgctga
agtttgtccc taaaggcaaa 421 gaaacttctg
cctcccaggg tcactaggcg ggcagcccac acccacccca
gacggccacc 481 acactgaggc cacacgttgg
ccattccacc ttggagttgg aaccctgggc gtcgagacag
541 gaaggcaggg cgcagtggtt gaaacatcag gacactccca
aggccccggc tctgaacaag 601 accttttcgt
ttcttggaaa agagactcat ttgctgatgg ttcatgcctt
ctgctgggac 661 aggcctgggc tgtgcagcca
cactgtcggc tgacttagcc ccctgctcac tctaggtgcc
721 tccaggaggt gagccctggg tgcagctggt ctctgaatga
cgttacaccc tcaccttctt 781 ttcctggccc
tgtctctgga ctctcccctg tgaggcccaa ttccaagaca
gactctcgtc 841 ctcaccgaag cttaggccca
catctcccag gctgcttagg agacagaatg gaaacggagg
901 ccgcccctgc cagccgccct ggccctggtc actgcatgat
ccgctctggt caaacccttc 961 caggccagcc
agagtgggga tggtctgtga cctgctggga aggcaggctg
atggggcaca 1021 cccttggcct ctcgtccacg
aggggagaaa cctaaaccct gtttcacaat ctgtgcggaa
1081 gtagcttgcc tcacttctgc ttaggaaagc ggctgttgct
ccataactct aaccagcaca 1141 gggctgaggc
ctgcagtgca cacctgcagg gaggcccttc ccaaggtgtg
gtgactgtgc 1201 cttactgtac atgctcggag
gcctggccat ataggagggt gggtgatgct gaaatcaccc
1261 cccatcttaa gtaattactt tctggagtaa tcaggtggaa
atccatagac aaatgaaaaa 1321 aaaaaaaaaa
aaaaaaa
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???? ?????? ?? SECIS selenoprotein X ????
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????? ??? SECIS ?? selenoprotein ??????


Score E Sequences producing
significant alignments
(bits) Value gi7706510re
fNM_016332.1 Homo sapiens selenoprotein X,
1... 123 1e-26 gi13111912gbB
C003127.1BC003127 Homo sapiens, Similar to...
123 1e-26 gi6649218gbAF166124.1A
F166124 Homo sapiens selenoprotei...
123 1e-26 gi11878031embAL499629.1AL499629
Human DNA sequence from.. . 123
1e-26 gi3367511gbAC005363.1AC005363 Homo
sapiens chromosome 1... 123
1e-26 gi10438878dbjAK026128.1AK026128 Homo
sapiens cDNA FLJ2... 123
1e-26 gi7305478refNM_013759.1 Mus musculus
selenoprotein R (S... 46
0.003 gi6470252gbAF195142.1AF195142 Mus
musculus selenoprotei... 46
0.003
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alignment ??? SECIS ?? selenoprotein X ?-
Selenoprotein R
gtgi7305478refNM_013759.1 Mus musculus
selenoprotein R (Sepr), mRNA Length
881 Score 46.1 bits (23), Expect 0.003
Identities 41/47 (87) Strand Plus / Plus

Query 8 atgatccgctctggtcaaacccttccaggcca
gccagagtggggatg 54

Sbjct 469
atgatctgctctggtcaaatccttctatgccagccagggtggtgatg
515
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alignment ??? ??? ?????? ???? ?- cDNA ??
Selenoprotein R ?? MUS
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???? ?????? ?? SECIS ?? selenoprotein X ?- MUS
gtgi7305478refNM_013759.1 Mus musculus
selenoprotein R (Sepr), mRNA 1
cccggacctg gctgggagct tttgcctgtc ccgtcgcgac
catgtcgttc tgcagcttct 61 tcggaggcga
ggttttccag aatcacttcg agccaggtgt ctacgtgtgt
gccaagtgca 121 gctatgagct gttctccagt
cactcgaagt acgcacactc atccccgtgg ccagcgttca
181 ctgaaaccat ccacccagac agtgtgacca agtgccctga
gaaaaaccga ccagaagctt 241 taaaggtgtc
ctgtggcaag tgtggcaatg ggttgggcca cgagttcctg
aatgatggcc 301 ccaagcgggg acaatcaaga
ttctgaatat ttagcagctc actgaagttc gtccctaaag
361 gcaaagaagc tgctgcctcc caggggcact aggacatgga
ctgcgaatgg cctgccccct 421 gccccctgcc
ccctgtcccc tgccaaccgc tccagcctca gtcaccgaat
gatctgctct 481 ggtcaaatcc ttctatgcca
gccagggtgg tgatgacccg tgaccttttg aggaggggag
541 taggctgata ccgggaactc tttggatcct tgccttctgg
cagagaaaac aaaactccgt 601 ttcataatct
gcaaactcat cttgcctcac tctagcttgg ggaagtgaca
gttgttgccc 661 cattagctgg ccagcgtggc
agggaccctc ttctcaccac tttgtccagc gtgtacctcc
721 caagagaaag ccactgtacc ttattggtgt gtttggaagc
tcttgacacc tgggagagtg 781 gctgaagctg
agacccactc ccccccttga ttgctctctg ggatgatcaa
gtagaagtca 841 ataaacatgc agcctgagcc
gttaaaaaaa aaaaaaaaaa a
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?????? ?? MFOLD????? ?????? ?? selenoprotein X
????
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????? ???? ?????? ??? SECIS ?????? ?-
selenoprotein X
??????????
Homosepiens Selnoprotein T
Mus selenoprotein R
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????? ???? ?????? ??? SECIS ?????? ?-
selenoprotein X
??????????
Clostridium purinolyticum selenoprotein A
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?????

• ?????? ????? ???? ???? ???? ??
• SECIS

• ????? ???? ??????? ?? ???? ?-
• SECIS ?????? ????? ????? ??????
• ???????? ?? ????? ???????