Dr. Mark F. Feldlaufer

1
2007 DoD Pest Management Workshop
Deployed War Fighter Protection (DWFP) Research
Program
CAIBL activities
photo by J. Gathany
Dr. Mark F. Feldlaufer Research Leader Chemicals
Affecting Insect Behavior Laboratory
(CAIBL) Beltsville Maryland
2
CAIBL
Manipulation of Arthropod Behavior For Protection
of Humans
Chemical Signals for Managing Insects
4 entomologists 3 organic chemists
3
against
Master Memorandum of Understanding (MOU) DWFP
is an initiative to develop and validate novel
methods to protect US military deployed abroad
from threats posed by disease-carrying insects.
The DWFP is designed not only to encourage the
rapid development of such products but to improve
the capability of USDA to provide long term,
innovative support to military preventive
medicine.
4
reformulation of existing product
Development and Reformulation of IR3535-Based
Repellents
(IR3535 - Ethyl butylacetylaminoproprionate)
AVONS SKIN SO SOFT BUG GUARD PLUS IR3535
10 lotion 20 pump spray 20 aerosol
Supplying CAIBL with neat IR3535 blank
formulations formulated compounds
GOALS superior product facilitate military
availability
5
against
Master Memorandum of Understanding (MOU) DWFP
is an initiative to develop and validate novel
methods to protect US military deployed abroad
from threats posed by disease-carrying insects.
The DWFP is designed not only to encourage the
rapid development of such products but to improve
the capability of USDA to provide long term,
innovative support to military preventive
medicine.
6
discovery of new products
BASF supplies CAIBL with chemicals from their
library
CAIBL confirms identity and checks purity
CAIBL prepares stock solutions for testing

CAIBL conducts bioassays / statistical analyses
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BIOASSAY
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KD module -
four TEST compounds (25nM/cm2) solvent control
(acetone) positive control (a-cypermethrin
standard) five mosquitoes/chamber (12 reps)
blood fed or not blood fed compounds ranked

Category 1 no statistical difference from
solvent control INACTIVE
Category 2 statistical difference from both
Category 3 no statistical difference from
a-cypermethrin standard ACTIVE
9

- discovery of new products
USUHS (Uniformed Services University of the
Health Sciences) Kasetsart University (Thailand)
10
discovery of new products
BASF supplies CAIBL with chemicals from their
library
CAIBL confirms identity and checks purity
CAIBL prepares stock solutions for testing

CAIBL conducts bioassays / statistical analyses
USUHS (Uniformed Services University of the
Health Sciences) Don Roberts John
Grieco Nicole Achee
11
Two additional in vitro mosquito assays
measures (i) Contact irritancy (CIA)
(ii) Spatial Repellency (SRA)
Ae. aegypti (Thai Houston) Culex pipiens An.
albimanus An. minimus
12
Kasetsart University (Thailand) Kanchanaburi Chai
ng Mai
13

- discovery of new products
KD
KD CIA
KD CIA
10
KD CIA SRA
4
Chemical Optimization Dose titration (25
nM/cm2) Mode of action Cost Registerability
14
against
Master Memorandum of Understanding (MOU) DWFP
is an initiative to develop and validate novel
methods to protect US military deployed abroad
from threats posed by disease-carrying insects.
The DWFP is designed not only to encourage the
rapid development of such products but to improve
the capability of USDA to provide long term,
innovative support to military preventive
medicine.
15
(No Transcript)
16
Wind Tunnel research Dr. Jerry Klun attraction
human breath odors with and without CO2
17
(No Transcript)
18
CAIBL
Manipulation of Arthropod Behavior For Protection
of Humans
Chemical Signals for Managing Insects
4 entomologists 3 organic chemists
19
Electrophysiology of the CPB
20
Dick Dickens, Principal Investigator with
Meiling Webb, Support Scientist
Sensillum
Microelectrode
Antenna (Nose)
Dr. J. C. Dickens, BARC, CAIBL, Beltsville, MD
2007
21
photo by E. Erbe
22
against
Master Memorandum of Understanding (MOU) DWFP
is an initiative to develop and validate novel
methods to protect US military deployed abroad
from threats posed by disease-carrying insects.
The DWFP is designed not only to encourage the
rapid development of such products but to improve
the capability of USDA to provide long term,
innovative support to military preventive
medicine.
23
Chemical Affecting Insect Behavior Laboratory
SCIENTISTS Jeff Aldrich Kamal Chauhan Dick
Dickens Mark Feldlaufer Ashot Khrimian Jerry
Klun Kevin Tran Aijun Zhang
SUPPORT STAFF John Davis Blanca Evans Fil
Guzman Sandra Lara (s) Matt Lawrence June
Nie Deborah Smith Meiling Webb
OFFICE Julia Cypriano Kawal Sethi
DWFP - funded