Fullface NonContact Chromophore Mapping using SIAscopy is a new technique that generates melanin and

1
P 219
The Use of Chromophore Mapping to Measure the
Effects of a Topical N-Acetyl Glucosamine /
Niacinamide Complex on Pigmentation in Human Skin
P J Matts PhD1, K Miyamoto PhD2, D L Bissett PhD2
and S D Cotton PhD3, The Procter Gamble
Company, Egham, UK1, Cincinnati, USA2 and Astron
Clinica, Cambridge, UK3
Finally, analysis of the NCS hemoglobin maps
demonstrated no difference between the vehicle
control and NAGN, confirming the lack of
irritation for the NAGN combination observed via
other clinical observations.
RESULTS
OBJECTIVE
INTRODUCTION
NCS analysis yielded a set of melanin and
hemoglobin grayscale concentration maps in
addition to the original high-resolution color
images (see below for examples). The inherent
advantage of the technique is illustrated below
where it is evident that ? there is excellent
melanin and hemoglobin separation recombination
of these maps explains observed skin tone in the
color image ? the apparent lightening of skin
tone and pigmented spots can be attributed to
changes in the melanin chromophore
Analysis of the NCS melanin maps demonstrated
clear treatment effects for the NAG N
combination vs vehicle control, resulting in a
significant (plt0.05) reduction in spot area
fraction and a significant (plt0.05) increase in
melanin evenness (Figures 2 and 3 below).
Full-face Non-Contact Chromophore Mapping using
SIAscopyä is a new technique that generates
melanin and hemoglobin parametric concentration
maps1,2,3. These grayscale maps can be analyzed
to provide quantitative data relating to
chromophores changes over time or in response to
treatment. The technique (termed NCS) was
deployed in a vehicle-controlled clinical study
to assess its utility as a measure of the effects
of N-acetyl glucosamine (NAG) / niacinamide (N)
in mitigating facial hyper-melanised pigmented
spots.
Evaluate the utility of full-face Chromophore
Mapping as a new clinical tool to measure the
effects of topical N-acetyl glucosamine in
combination with niacinamide on the appearance of
facial hyperpigmented spots.
CONCLUSIONS

• NCS non-contact, full-face chromophore mapping
  and analysis is able to detect and quantify
  changes in melanin concentration and distribution
  independent of hemoglobin
• Changes in skin tone observed by use of N-acetyl
  glucosamine niacinamide formulations can be
  attributed to a reduction in both hyper-melanised
  spot size and heterogeneity of melanin
  distribution, with associated visual benefits.

p0.21
Mean change in melanin spot area fraction
METHODS
Figure 1 Example images of subject who received
NAGN
p0.026



Overall p0.049
p0.053
A double-blind, vehicle-controlled, full-face
clinical study was performed in which subjects (n
200, ages 40-60) with facial hyper-pigmentation)
used one of of two facial treatment regimens.
After a 2-week wash-out period (all subjects
using the same facial cleanser and moisturizer
products), subjects applied their
randomly-assigned products to their faces for 8
weeks as follows ? 100 subjects used an SPF-15
moisturizing sunscreen lotion product
(daytime) and a moisturizer cream
(night-time) ? 100 subjects used an SPF-15
moisturizing sunscreen lotion product which
contained 2 NAG 4 niacinamide (N) (daytime)
and a moisturizer cream which contained 2 NAG
4 N (night-time).
At 0, 4, 6, and 8 weeks, high-resolution digital
facial images were captured using a
specially-calibrated imaging rig (calibrated 6.2
megapixel Fuji S2 Pro digital SLR and calibrated
custom flash source), allowing subsequent
processing of .RAW images to yield exclusive
melanin and hemoglobin grayscale concentration
maps. Regions of interest (ROI) within melanin
maps of the cheek area were analyzed using custom
algorithms to yield two principal endpoints ?
spot area fraction (total area occupied by
melanin spots as a percentage of the whole
ROI) ? an index of evenness of melanin
distribution
Vehicle NAG N
Vehicle control
Figure 2 change in melanin spot area fraction
vs baseline
References 1. Moncrieff, M., Cotton, S.,
Claridge, E. and Hall, P., Spectrophotometric
intracutaneous analysis - a new technique for
imaging pigmented skin lesions, British Journal
of Dermatology, 2002, 146(3), 448-457 2. Matts.
P.J., Carey, J. and Cotton, S.D., Chromophore
mapping a new technique to characterise ageing
human skin, in vivo, American Academy of
Dermatology 63rd Annual Conference,
2005 3. Preece, S., Cotton, S.D. and Claridge,
E, Imaging the pigments of skin with a technique
which is invariant to changes in surface geometry
and intensity of illuminating light, Medical
Image Understanding and Analysis, 2003, D. Barber
(Ed.), 145-148
Original color image, baseline
Melanin map, baseline
Hemoglobin map, baseline
p0.16
Mean change in melanin evenness (lower better)
p0.016


p0.019

Overall p0.028
Vehicle NAG N
Vehicle control
Original color image, 8 weeks
Melanin map, 8 weeks
Hemoglobin map, 8 weeks
This work was supported in its entirety by
Procter Gamble.
Figure 3 change in melanin evenness vs baseline
(lower more even)