??? ??????? Genetic Engineering in Yeast

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??? ???????Genetic Engineering in Yeast
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Biological Characteristics of the Bakers Yeast

• They grow quickly, doubling in about 2 hours
  (16-24 hours in mammalian cells).
• The yeast genome is very small .
• Yeast is especially suited for genetic analysis
  (either as haploid or diploid).
• As eukaryotes, they are organized much like cells
  in more complex organisms.

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The size of the genome in selected organisms
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Classes of yeastvectors

• I integrating
• E episomal
• R replicating
• C centromere

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Shuttle vector
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Yeast selectable markers
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Cloning of yeast biosynthetic genes by
comlementation in E.coli
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Cloning a yeast gene by geneticcomplementation
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Replica plating
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Gene targeting by homologous recombination
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Replacing a chromosomal gene by transplacement
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Tetrad Analysis to Detect Linkage in Fungi
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Tetrad analysis (gene knockout)
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The pheromonesignaling pathway
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Cloning of the gpa1 gene
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The receptor swap experiment
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Genetic demonstration that U2 RNA base-pairs with
an intron sequence
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A genetic assay for protein-protein interaction
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Plasmid shuffle