Effects%20of%20Drugs%20on%20Daphnia - PowerPoint PPT Presentation
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Effects%20of%20Drugs%20on%20Daphnia
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Effects of Drugs on Daphnia Michelle Dilgard Undergraduate Student Education Major Biology Concentration Tennessee Technological University Cookeville, TN 38505 – PowerPoint PPT presentation
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Title: Effects%20of%20Drugs%20on%20Daphnia
1
Effects of Drugs on Daphnia
- Michelle Dilgard
- Undergraduate Student
- Education Major
- Biology Concentration
- Tennessee Technological University
- Cookeville, TN 38505
2
Project Summary
- Problem How do drugs effect Heart Rate?
- Using Daphnia is more appropriate
- Through the performing of an experiment
3
Introduction and Background
- Daphnia
- Water Flea (Wong, 1997)
- Many studies have been done previously
- Drugs to be used
- Caffeine (Foster, 1997)
- Nicotine
- Alcohol
- Ibuprofen (Cluevers, 2003)
4
Objective
- I will observe the changes in heart rate of
Daphnia when exposed to Caffeine, Ibuprofen,
Alcohol, and Nicotine. I will have several
separate samples of Daphnia in my study. Each
community will be exposed to a different drug and
observe how the heart rate of the Daphnia changes
accordingly.
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Hypothesis
- I believe the Daphnia heart rate will increase
when the Daphnia are exposed to Caffeine and
Nicotine. I believe the Daphnia heart rate will
decrease and slow down when the Daphnia are
exposed to Ibuprofen and Alcohol.
6
Methods
- Label containers
- Place Daphnia on slide
- Record Heart Rate
- Add experimental solution
- Record new heart rate
- Repeat
Data Form
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Materials
- Water
- Depression Slides
- Cover slips
- Droppers
- Daphnia Magna
- Microscope
- Watch
- Petri Dishes
- Daphnia Anatomy Chart
- Cigarettes
- 80 proof vodka
- No-dos
- Ibuprofen tablets
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Expected Results
- I expect that the Daphnias heart rates will
increase with Nicotine and Caffeine because both
of these drugs are stimulants. - I also expect that the Daphnias heart rates will
decrease with Alcohol and Ibuprofen solution.
9
Benefits
- This experiment will show not only the effects of
these drugs on Daphnia but also what they
similarly do to the human body when it is
exposed. - Daphnia are used as a humane alternative to
Humans when performing this type of experiment.
10
Timeline
- 9-6-06 Title Due
- 9-13-06 Literature Reviews and Works Cited Due
- 9-20-06 Method Objective and Hypothesis Due
- 9-27-06 Research Proposal
- 10-4-06 Website
- 10-11-06 Midterm Due
- 10-25-06 Begin Experiment
- 12-6-06 Final Presentation
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Budget
- Petri Dishes- 5.10
- Slides- 7.50
- Cover Slips- 3.20
- Daphnia- 10.95
- Caffeine Pills- 7.49
- Ibuprofen- 9.99
- Cigarettes- 3.40
- Vodka- 16.99
- Pipettes- 10.00
- TOTAL 74.62
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Works Cited
- Campbell, Anthony K., Kenneth T. Wann, and
Stephanie B. Matthews. 2004. Lactose causes
heart arrhythmia in the water flea Daphnia pulex.
Comparative Biochemistry and physiological Part
B Biochemistry and Molecular Biology 139
225-234. - Cluevers, Michael. 2004. Mixture toxicity of the
anti-inflammatory drugs diclofenac, ibuprofen,
naproxen, and acetylsalicylic acid.
Exotoxicology an Environmental Safety 59
309-315. - Foster, Rachel. 1997. A stroboscopic method to
investigate the effect of caffeine on Daphnia
hear rate. Journal of Biological Education 31
253-255. - Wong, Diana C.L., Philip B. Dorn, and Eric Y.
Chai. 1997. Acute toxicity and
structure-activity relationships of nine alcohol
ethoxylate surfactants to fathead minnow and
Daphnia magna. Environmental Toxicology and
Chemistry 16 1970-1976. - Wang, Helen Ying, Allen W. Olmstead, Hong Li, and
Gerald A. LeBlanc. 2005. The screening of
chemicals for juvenoid-related endocrine activity
using the water flea Daphnia magna. Aquatic
Toxicology 74 193-204. - Villegas-Navarro, Arturo, Esperanza L. Ross, and
Jose L. Reyes. 2003. The heart of Daphnia
magna effects of four cardioactive drugs.
Comparative Biochemisty and Physiology Part C
Toxicology and Pharmacology 136C 127-134.
