SCERP Project A051: Cytotoxicity Studies Relating to Long Term Exposure to Low Concentrations of Car - PowerPoint PPT Presentation

Title: SCERP Project A051: Cytotoxicity Studies Relating to Long Term Exposure to Low Concentrations of Car


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SCERP Project A-05-1 Cytotoxicity Studies
Relating to Long Term Exposure to Low
Concentrations of Carbons Nanotube Aggregates in
the Indoor Air Implications for Asthma and Other
Respiratory Health Effects in the Paso Del Norte
Region L. E. Murr, P.I.

Department of Metallurgical and
Materials Engineering
The University of Texas at El Paso, El
Paso, TX 79968 K. M. Garza, Co-P.I.

Department of Biological Sciences

The University of Texas at
El Paso, El Paso, TX 79968 Presented By
Priscilla Guerrero and Ariana Guerrero
Department of
Metallurgical and Materials Engineering
The University of Texas
at El Paso, El Paso, TX 79968
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(a) Schematic view of high-volume collector
arrangement for a natural gas (range) burner. (b)
Top view of natural gas cook top burner shown
schematically in (a). G shows gas inlet reference.
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Views of the high-volume PM collection from the
natural gas burner in previous slide. (a) Overall
view of the collection system. (b) Close up
showing viewing window. (c) High-flow blue flame
(HFBF) viewed through the window in (b).
(d) Thermal precipitator (TP) situated at the end
(or top) of the collection line (removed from the
high-volume collector) for PM collection for TEM
analysis. IW is the input cooling water container
for the TP collector block.
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(c) FESEM-SE image of (b).
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TEM images of natural gas soot PM
(a) Black carbon (BC) placed on Tem grid sandwich.
(d), MWCNT aggregates collected above a kitchen
stove during natural gas, by thermal
precipitation.
(b) Soot collected by electrostatic
precipitation,
(c) Commercial MWCNT aggregate material.
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Comparative PAH content () and total PAH content
for carbonaceous PM (underlined numbers in mg/g).
The PAH key is as follows (from MW 128-278).
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Conclusion

• In this short report, we have illustrated a new
  methodology for at least a qualitative assessment
  and comparison of cytotoxic responses for a
  variety of environmental soots, and soot-related
  nanoparticles/aggregates. This involves direct
  filter collections and cell contact. It is also
  apparent that while there are differences in the
  cytotoxic response of human epithelial (lung
  function surrogate) cell (A549), detailed
  microstructural characterization shows that the
  soots appear to be identical, same for
  variations in the primary particle (spherule
  nanoparticle) size (or size distribution).

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Accomplishments

• Publications (Published, Accepted (In Press),
  Submitted)
• Combustion-Generated Nanoparticles in the El
  Paso, TX, USA/Juarez, Mexico Metroplex Their
  Comparative Characterization and Potential for
  Adverse Health Effects, L.E Murr, K.F. Soto,
  K.M. Garza, P.A. Guerrero, F. Martinez, E.V.
  Esquivel, D.A. Ramirez, Y. Shi, J.J. Bang, J.
  Venzor, III, International Journal of
  Environmental Research and Public Health (2006),
  Vol. 3(1), pp.48-66,
• Cytotoxic Response for Carbon Nanotubes and
  Aggregates and Other Manufactured Nanoparticles
  The Role of In Vitro Biological Assays in
  Nanomaterials Evaluations, K.F. Soto, K.M.
  Garza, L.E. Murr, Acta Biomaterialia, revised
  submission (2006) in press.
• Carbon Nanotubes in Wood Soot, L.E. Murr and
  P.A. Guerrero, Atmospheric Science Letters
  (2006), published on-line in Wiley Interscience,
  in press.

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Questions?
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