A1257278316fZLhS - PowerPoint PPT Presentation

Title: A1257278316fZLhS


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Louis Pasteur watches a rabies vaccination
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Immunity 2 Branches 1. Non-specific Immunity
Examples?
2. Specific Acquired Immunity
Specific
Has memory
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Mucosal membrane blocks entry of E. coli from
urinary tract.
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RBC
monocyte
Macrophage attacking E. coli
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Chapter 2 Cells and Organs of the Immune
System
A macrophage interacting with bacteria
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Inflammation
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Adaptive Immunity 2 Branches
Humoral
Cell-mediated
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T-lymphocyte interacting with a macrophage
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Overview of Immune System
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MHC interactions
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Response to Ag (humoral system)
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Response to Ag (CMI system)
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Electrophoresis of serum proteins
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Generic Ab
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Historical experiments for structure
determinations.
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Ribbon diagram of a monoclonal ab
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These ab classes have an additional domain which
replaces the hinge region
Domain structure of abs
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Light chain ribbon diagram
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IgG
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Variability of residues in different abs. The 3
hypervariable regions are termed
complementarity- determining regions (CDR)
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Side-view of Angiotensin II bound to Fab.
Van der Waals surface contacts
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A peptide fomr the HIV protease with an
anti-protease Fab
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Ab classes
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Four IgG subclasses
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IgA
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Allergic Response
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Allergen cross-linking of receptor bound IgE on
mast cells
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Antigenic determinants of Abs
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mIg membrane bound Ig Each mIg associates with
one heterodimer of Ig-? and Ig-?
B-cell receptor (BCR)
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Structures of human Fc receptors
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The Ig superfamily
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Polyclonal ab production
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Organization and Expression of Ig Genes
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From Hozumi and Tonegawa, 1976, PNAS USA 73 3628
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(human chromosome 22)
(human chromosome 2)
(human chromosome 14)
Gene arrangements
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Kappa gene expression
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H-gene expression
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Recombination signal sequences
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Circular DNA isolated from thymocytes undergoing
TCR rearrangement
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When ss DNA is generated
Extra nucleotides can also be added
Nucleotide addition during joining
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Somatic variation
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Allelic exclusion (only in B and T cells)
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Model of allelic exclusion
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Class switching
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Secreted vs. membrane forms of IgM
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Class switching by alternative processing
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Secretory pathway
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cis-acting sites
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